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Product Info Summary
| SKU: | M05806 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-hnRNP F (HNRNPF) Mouse Monoclonal Antibody [Clone ID: OTI5F5]
SKU/Catalog Number
M05806
Size
100 µl
Description
Boster Bio HNRNPF (hnRNP F) mouse monoclonal antibody, clone OTI5F5 (formerly 5F5). Catalog# M05806. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-hnRNP F (HNRNPF) Mouse Monoclonal Antibody [Clone ID: OTI5F5] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05806)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI5F5
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human HNRNPF (NP_004957) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05806 is reactive to HNRNPF in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
46 kDa
Calculated molecular weight
45.672kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05806 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HNRNPF using anti-HNRNPF antibody (M05806).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SK-OV-3 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human PANC-1 whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HNRNPF antigen affinity purified monoclonal antibody (Catalog # M05806) at at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HNRNPF at approximately 46 kDa. The expected band size for HNRNPF is at 46 kDa.
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT) and HNRNPF-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-HNRNPF monoclonal antibody M05806
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Anti-HNRNPF mouse monoclonal antibody (M05806) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HNRNPF.
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Immunofluorescent staining of HepG2 cells using anti-HNRNPF mouse monoclonal antibody (M05806).
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Flow cytometric Analysis of Hela cells
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Flow cytometric Analysis of Jurkat cells
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HNRNPF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HNRNPF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HNRNPF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-HNRNPF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HNRNPF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNRNPF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HNRNPF.
Protein Target Info & Infographic
Gene/Protein Information For HNRNPF (Source: Uniprot.org, NCBI)
Gene Name
HNRNPF
Full Name
Heterogeneous nuclear ribonucleoprotein F
Weight
45.672kDa
Alternative Names
heterogeneous nuclear ribonucleoprotein F; hnRNP F; HNRPFHnRNP F protein; mcs94-1; MGC110997; Nucleolin-like protein mcs94-1; OK/SW-cl.23 HNRNPF HNRPF, OK/SW-cl.23, mcs94-1 heterogeneous nuclear ribonucleoprotein F heterogeneous nuclear ribonucleoprotein F|HnRNP F protein|nucleolin-like protein mcs94-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HNRNPF, check out the HNRNPF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HNRNPF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-hnRNP F (HNRNPF) Mouse Monoclonal Antibody [Clone ID: OTI5F5] (M05806)
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