Click image to see more details
-
-
-
-
-
+5
Product Info Summary
| SKU: | PA1769 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, IHC-F, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-MCM6 Antibody Picoband®
SKU/Catalog Number
PA1769
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MCM6 Antibody catalog # PA1769. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MCM6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1769)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human MCM6, different from the related rat and mouse sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1769 is reactive to MCM6 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
93 kDa
Calculated molecular weight
92889 MW
Background of MCM6
MCM6 (Minichromosome maintenance, s. pombe, homolog of, 6) is a protein that in humans is encoded by the MCM6 gene. MCM6 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The MCM genes were originally identified in yeast defective in minichromosome maintenance and have since been shown to play roles in the progression of the cell cycle; many are cell division control genes. The MCM6 gene is mapped on 2q21.3. Mcm 6 has recently been shown to interact strongly Cdt1 at defined residues, by mutating these target residues Wei et al. observed lack of Cdt1 recruitment of Mcm2-7 to the pre-RC. An approximately 200-kb region surrounding the C/T (-13910) polymorphism in MCM6 intron 13 functioned as an enhancer of the lactase gene promoter in intestinal cell culture.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1769 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, Rat, Mouse
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: U87 Cell, COLO320 Cell, HELA Cell, MCF-7 Cell, JURKAT Cell,, mouse small intestine tissue, mouse spleen tissue,
IHC: Human Intestinal Cancer Tissue, Rat Brain Tissue
IHC-F: Human Placenta Tissue, rat intestine tissue
ICC/IF: A431 cell
ICC: HELA Cell
FCM: SiHa cell
Validation Images & Assay Conditions
Click image to see more details
Anti-MCM6 antibody, PA1769, Western blotting
Lane 1: U87 Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: MCF-7 Cell Lysate
Lane 5: JURKAT Cell Lysate
Click image to see more details
Anti-MCM6 antibody, PA1769, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Click image to see more details
Anti-MCM6 antibody, PA1769, IHC(P)
IHC(P): Rat Brain Tissue
Click image to see more details
Anti-MCM6 antibody, PA1769, ICC
ICC: HELA Cell
Click image to see more details
Anti-MCM6 antibody, PA1769, IHC(F)
IHC(F): Human Placenta Tissue
Click image to see more details
Figure 6. Western blot analysis of MCM6 using anti-MCM6 antibody (PA1769).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse small intestine tissue lysates,
Lane 2: mouse spleen tissue lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM6 antigen affinity purified polyclonal antibody (Catalog # PA1769) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCM6 at approximately 93KD. The expected band size for MCM6 is at 93KD.
Click image to see more details
Figure 7. IHC analysis of MCM6 using anti-MCM6 antibody (PA1769).
MCM6 was detected in frozen section of rat intestine tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MCM6 Antibody (PA1769) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 8. IF analysis of MCM6 using anti-MCM6 antibody (PA1769).
MCM6 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-MCM6 Antibody (PA1769) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 9. Flow Cytometry analysis of SiHa cells using anti-MCM6 antibody (PA1769).
Overlay histogram showing SiHa cells stained with PA1769 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCM6 Antibody (PA1769, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For MCM6 (Source: Uniprot.org, NCBI)
Gene Name
MCM6
Full Name
DNA replication licensing factor MCM6
Weight
92889 MW
Superfamily
MCM family
Alternative Names
DNA replication licensing factor MCM6;3.6.4.12;p105MCM;MCM6; MCM6 MCG40308, Mis5, P105MCM minichromosome maintenance complex component 6 DNA replication licensing factor MCM6|MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe)|minichromosome maintenance deficient (mis5, S. pombe) 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MCM6, check out the MCM6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MCM6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MCM6 Antibody Picoband® (PA1769)
Hello CJ!
No publications found for PA1769
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-MCM6 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-MCM6 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-MCM6 Antibody Picoband®
Question
Is a blocking peptide available for product anti-MCM6 antibody (PA1769)?
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
We do provide the blocking peptide for product anti-MCM6 antibody (PA1769). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-03
Question
Would anti-MCM6 antibody PA1769 work for ICC with brain?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
According to the expression profile of brain, MCM6 is highly expressed in brain. So, it is likely that anti-MCM6 antibody PA1769 will work for ICC with brain.
Boster Scientific Support
Answered: 2019-12-13
Question
Will anti-MCM6 antibody PA1769 work on goat IHC-F with cervix?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
Our lab technicians have not validated anti-MCM6 antibody PA1769 on goat. You can run a BLAST between goat and the immunogen sequence of anti-MCM6 antibody PA1769 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat cervix in IHC-F, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-27
Question
We are currently using anti-MCM6 antibody PA1769 for rat tissue, and we are satisfied with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
The anti-MCM6 antibody (PA1769) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-23
Question
Do you have a BSA free version of anti-MCM6 antibody PA1769 available?
E. Bhatt
Verified customer
Asked: 2017-06-27
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MCM6 antibody PA1769 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-06-27
Question
I have attached the WB image, lot number and protocol we used for brain using anti-MCM6 antibody PA1769. Please let me know if you require anything else.
L. Brown
Verified customer
Asked: 2015-04-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-04-08
Question
Is this PA1769 anti-MCM6 antibody reactive to the isotypes of MCM6?
C. Yang
Verified customer
Asked: 2015-03-19
Answer
The immunogen of PA1769 anti-MCM6 antibody is A synthetic peptide corresponding to a sequence in the middle region of human MCM6(589-605aa ESEDFIVEQYKHLRQRD), different from the related rat and mouse sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-03-19
