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Product Info Summary
| SKU: | P05108 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-FANCI (Ser556) Antibody
SKU/Catalog Number
P05108
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-FANCI (Ser556) Antibody (Catalog # P05108). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-FANCI (Ser556) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P05108)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser556 of human FANCI, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Human.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P05108 is reactive to FANCI in Human
Reconstitution
Observed Molecular Weight
52 kDa
Calculated molecular weight
149324 MW
Background of FANCI
Fanconi anemia, FA, is a rare disorder where cells cannot prevent, repair, or tolerate DNA damage, leading to cancer, progressive bone marrow failure and developmental abnormalities (Ishiai et al, 2008). 16 genes have been implicated in FA, and their products constitute a common FA pathway (Walden and Deans 2014). FANCI (Fanconi anemia complementation group I), is one of two substrates for monoubiquitination by the FANCL-containing core complex, and is crucial for DNA repair via FAP and intrastrand cross-links (Walden and Deans, 2014). Phosphorylation at Ser-556 has been shown to play a key role in FANCI physically associating with FANCD2, stabilizing the ID2 complex, and supporting its DNA binding and subsequent D2-ubiquitination (Walden and Deans, 2014).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P05108 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
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Western blot of HeLa cell lysate showing specific immunolabeling of the ~150 kDa FANCI protein phosphorylated at Ser556 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (gamma-Ptase, 800 units/1mg protein for 30 min).
Protein Target Info & Infographic
Gene/Protein Information For FANCI (Source: Uniprot.org, NCBI)
Gene Name
FANCI
Full Name
Fanconi anemia group I protein
Weight
149324 MW
Alternative Names
FANCI antibody, FANCI gene antibody, FANCI_HUMAN antibody, Fanconi anemia group I protein antibody, Fanconi anemia complementation group I antibody, FLJ10719 antibody, FLJ14658 antibody, KIAA1794 antibody, Protein FACI antibody, Protein FANCI antibody, FANCI KIAA1794 FA complementation group I Fanconi anemia group I protein|Fanconi anemia complementation group I
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FANCI, check out the FANCI Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FANCI: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-FANCI (Ser556) Antibody (P05108)
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No publications found for P05108
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Customer Q&As
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1 Customer Q&As for Anti-Phospho-FANCI (Ser556) Antibody
Question
We are currently using anti-Phospho-FANCI (Ser556) antibody P05108 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
The anti-Phospho-FANCI (Ser556) antibody (P05108) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-27
