Mouse Meteorin-like/METRNL ELISA Kit PicoKine®

Meteorin-like/METRNL ELISA kit for Mouse

Mouse Meteorin-like/METRNL ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Metrnl in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Info Summary

SKU: EK1550
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse Meteorin-like/METRNL ELISA Kit PicoKine®

View all Meteorin-like/METRNL ELISA kits

SKU/Catalog Number

EK1550

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse Meteorin-like/METRNL ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Metrnl in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse Meteorin-like/METRNL ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1550)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: Q46-E311

Sensitivity

<12 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

See datasheet of EK1550 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1550 is reactive to Metrnl in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1550 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Meteorin-like/METRNL

METRNL (Meteorin-like) is a cytoplasmic 30 kDa (predicted) member of the meteorin family of proteins. It is expressed by neurons, and appears to function as a primer molecule that sets the stage for either cell differentiation or neurite outgrowth when it acts with Dclk1 and SerpinB1. In mice, exogenously elevated circulating Metrnl increased whole-body energy expenditure and improved glucose tolerance following diet-induced obesity. It also induced expression of antiinflammatory genes, leading to alternative activation of macrophages in adipose tissue and to browning of white fat depots. Metrnl did not appear to activate thermogenic genes directly, but it stimulated eosinophils and macrophages to enter adipose tissue and take their specific thermogenic actions. Blockade of cytokine signaling eliminated Metrnl-induced changes in gene expression required for alternative macrophage activation and blunted expression of beige fat thermogenic and beta-oxidation genes. It is concluded that METRNL is a hormone that influences energy expenditure and thermogenic programs in adipose tissue.

Kit Components

Catalog Number Description Quantity
EK1550-CAP Anti-Mouse Metrnl Pre-coated 96-well strip microplate 1
EK1550-ST Mouse Metrnl Standard 2 vials, 10 ng/tube
EK1550-DA Mouse Metrnl Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0100.0770.1260.2390.4410.8341.4411.986

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Meteorin-like/METRNL, Dilution ratio of 1:20, concentration in serum and plasma is 13.6-18.3 ng/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse Meteorin-like/METRNL ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)1806649771686641065
Standard deviation7.5639.1765.459.443.1674.55
CV (%)4.2%6.7%%6.7%5.6%6.5%7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Meteorin-like/METRNL in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11801801851861822.771.5%
Sample 266472969177071339.965.6%
Sample 397793410591080101259.455.8%
*number of samples for each test n=16.

Gene/Protein Information For Metrnl (Source: Uniprot.Org, NCBI)

Gene Name

Metrnl

Full Name

Meteorin-like protein

Weight

34530 MW

Superfamily

meteorin family

Alternative Names

Meteorin-like protein;Subfatin;Metrnl; Metrnl|9430048M07Rik, BC019776|meteorin, glial cell differentiation regulator-like|meteorin-like protein|cometin|subfatin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Metrnl, check out the Metrnl Infographic

Metrnl infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Metrnl: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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6 Customer Q&As for Mouse Meteorin-like/METRNL ELISA Kit PicoKine®

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

R. Zhao

Verified customer

Asked: 2020-11-22

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-11-22

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-09-19

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-09-19

Question

Q: how to analyze ELISA data? I have obtained Meteorin-like level in serum.

M. Jackson

Verified customer

Asked: 2020-08-08

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-08-08

Question

Q: What is the optimal O.D. value for Meteorin-like ELISA kit? I performed your Meteorin-like ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Meteorin-like even though the O.D. values are not very high?

M. Liu

Verified customer

Asked: 2020-06-03

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Meteorin-like ELISA kit to have sensitivity of 12pg/ml, that means the minimum amount of Meteorin-like that can be declared/interpreted as positive by the above standard is 12pg/ml.

Boster Scientific Support

Answered: 2020-06-03

Question

Q: can you recommend the dilution ratio of serum samples for detection of Meteorin-like in Mouse serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

R. Li

Verified customer

Asked: 2019-01-02

Answer

A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Meteorin-like ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-01-02

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Meteorin-like using the EK1550 Mouse Meteorin-like Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2016-09-12

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2016-09-12

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