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Product Info Summary
| SKU: | M00563 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-FANCD2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00563
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-FANCD2 Rabbit Monoclonal Antibody catalog # M00563. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FANCD2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00563)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GCE-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human FANCD2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00563 is reactive to FANCD2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
17 kDa
Calculated molecular weight
164128 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00563 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human MCF-7 whole cell
IHC: human spleen tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FANCD2 using anti-FANCD2 antibody (M00563).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FANCD2 antigen affinity purified monoclonal antibody (Catalog # M00563) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FANCD2 at approximately 164kDa. The expected band size for FANCD2 is at 164 kDa.
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Immunohistochemical analysis of paraffin-embedded human spleen, using FANCD2 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For FANCD2 (Source: Uniprot.org, NCBI)
Gene Name
FANCD2
Full Name
Fanconi anemia group D2 protein
Weight
164128 MW
Alternative Names
Fanconi anemia group D2 protein;Protein FACD2;FANCD2;FACD; FANCD2 FA-D2, FA4, FACD, FAD, FAD2, FANCD FA complementation group D2 Fanconi anemia group D2 protein|Fanconi anemia complementation group D2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FANCD2, check out the FANCD2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FANCD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FANCD2 Rabbit Monoclonal Antibody (M00563)
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No publications found for M00563
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1 Customer Q&As for Anti-FANCD2 Rabbit Monoclonal Antibody
Question
We are currently using anti-FANCD2 Rabbit Monoclonal antibody M00563 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2017-07-11
Answer
The anti-FANCD2 Rabbit Monoclonal antibody (M00563) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-11
