Anti-FANCD2 Antibody

Product Name

Anti-FANCD2 Antibody

View all FANCD2 Antibodies

SKU/Catalog Number

A00563-2

Size

100ul

Form

Liquid

Description

Boster Bio Anti-FANCD2 Antibody catalog # A00563-2. Tested in WB,ICC/IF,IHC,IP applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FANCD2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00563-2)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human AIRE-1 around the phosphorylation site of S156.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00563-2 is reactive to FANCD2 in Human, Mouse, Rat

Applications

A00563-2 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

164128 MW

Background of FANCD2

Transcriptional regulator that binds to DNA as a dimer or as a tetramer, but not as a monomer. Binds to G-doublets in an A/T-rich environment; the preferred motif is a tandem repeat of 5'-. ATTGGTTA-3' combined with a 5'-TTATTA-3' box. Binds to nucleosomes By similarity. Binds to chromatin and interacts selectively with histone H3 that is not methylated at 'Lys-4', not phosphorylated at 'Thr-3' and not methylated at 'Arg-2'. Functions as a sensor of histone H3 modifications that are important for the epigenetic regulation of gene expression. Functions as a transcriptional activator and promotes the expression of otherwise tissue-specific self-antigens in the thymus, which is important for self tolerance and the avoidance of autoimmune reactions.

Nagamine K., Nat. Genet. 17:393-398(1997).
Aaltonen J., Nat. Genet. 17:399-403(1997).
Hattori M., Nature 405:311-319(2000).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1,000
ICC: 1:100-1:500
IHC: 1:50-1:200

Validation Images & Assay Conditions

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Figure 1. Immunocytochemistry staining of FANCD2 using Anti-FANCD2 Antibody (A00563-2).

ICC staining FANCD2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde

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Figure 2. Immunocytochemistry staining of FANCD2 using Anti-FANCD2 Antibody (A00563-2).

ICC staining FANCD2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde

Gene/Protein Information For FANCD2 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp762A223; FA4; FACD; FACDFLJ23826; FAD; FAD2; FA-D2; FADFAD2; FANCD; FANCD2; Fanconi anemia group D2 protein; Fanconi anemia, complementation group D2; Protein FACD2 FANCD2 FA-D2, FA4, FACD, FAD, FAD2, FANCD FA complementation group D2 Fanconi anemia group D2 protein|Fanconi anemia complementation group D2

For more info on FANCD2, check out the FANCD2 Infographic

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In this infographic, you will see the following information for FANCD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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