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Product Info Summary
| SKU: | A00151Y397 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-FAK (phospho-Y397) PTK2 Antibody
SKU/Catalog Number
A00151Y397
Size
100ul
Form
Liquid
Description
Boster Bio Anti-FAK (phospho-Y397) PTK2 Antibody catalog # A00151Y397. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FAK (phospho-Y397) PTK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00151Y397)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phosphopeptide derived from human FAK around the phosphorylation site of Serine 397.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00151Y397 is reactive to PTK2 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
119233 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00151Y397 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of PTK2 using Anti-FAK (phospho-Y397) PTK2 Antibody (A00151Y397).
Immunohistochemistry (IHC) analyzes of p-FAK (Y397) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-FAK (phospho-Y397) PTK2 Antibody A00151Y397
Western blot (WB) analysis of FAK (phospho-Y397) polyclonal antibody at 1:500 dilutionLane1:CT26 whole cell lysate(40ug)
Lane2:PC12 whole cell lysate(40ug)
Lane3:A549 whole cell lysate(40ug)
Lane4:H1792 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For PTK2 (Source: Uniprot.org, NCBI)
Gene Name
PTK2
Full Name
Focal adhesion kinase 1
Weight
119233 MW
Superfamily
protein kinase superfamily
Alternative Names
Focal adhesion kinase 1;FADK 1;2.7.10.2;Focal adhesion kinase-related nonkinase;FRNK;Protein phosphatase 1 regulatory subunit 71;PPP1R71;Protein-tyrosine kinase 2;p125FAK;pp125FAK;PTK2;FAK, FAK1; PTK2 FADK, FAK, FAK1, FRNK, PPP1R71, p125FAK, pp125FAK protein tyrosine kinase 2 focal adhesion kinase 1|FADK 1|FAK-related non-kinase polypeptide|PTK2 protein tyrosine kinase 2|focal adhesion kinase-related nonkinase|protein phosphatase 1 regulatory subunit 71
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTK2, check out the PTK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FAK (phospho-Y397) PTK2 Antibody (A00151Y397)
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No publications found for A00151Y397
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