Key=store_id Key=entity_id, value=93680 Key=attribute_set_id, value=4 Key=type_id, value=simple Key=sku, value=A00237-4 Key=has_options, value=1 Key=required_options, value=1 Key=created_at, value=2020-10-22 02:22:20 Key=updated_at, value=2024-09-26 01:16:12 Key=mst_search_weight, value=0 Key=status, value=Enabled Key=name, value=Anti-FADD Antibody Picoband® Key=cj_search_index Key=publication_count, value=0 Key=qty_in_warehouses Key=is_recurring, value=No Key=visibility, value=Catalog, Search Key=tax_class_id, value=Taxable Goods Key=featured, value=No Key=featured_image, value=Yes Key=fb_product, value= Key=custom_product_page, value=No Key=free_shipping, value=No Key=template, value=antibodies Key=host, value=Rabbit Key=quantity_and_stock_status Key=googleshopping_exclude, value=No Key=yoast_keyword_score, value=-1189 Key=yoast_content_score, value=30 Key=badge, value=free-antibody-validation Key=price, value=370.0000 Key=weight, value=1.0000 Key=meta_title, value=Anti-FADD Antibody Picoband®| Bosterbio Key=meta_description, value=Boster Bio Anti-FADD Antibody Picoband® catalog # A00237-4. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. Key=image, value=/a/0/a00237-4-fadd-primary-antibodies-wb-testing-1.jpg Key=small_image, value=/a/0/a00237-4-fadd-primary-antibodies-wb-testing-1.jpg Key=thumbnail, value=/a/0/a00237-4-fadd-primary-antibodies-wb-testing-1.jpg Key=gallery, value=/A00237_1.jpg:/A00237_2.jpg:/A00237_3.jpg Key=url_key, value=anti-fadd-picoband-trade-antibody-a00237-4-boster Key=url_path, value=anti-hemoglobin-picoband-trade-antibody-a00233-1-boster Key=options_container, value=Product Info Column Key=image_label, value=Figure 1. Western blot analysis of FADD using anti-FADD antibody (A00237-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample Key=small_image_label, value=Figure 1. Western blot analysis of FADD using anti-FADD antibody (A00237-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample Key=thumbnail_label, value=Figure 1. Western blot analysis of FADD using anti-FADD antibody (A00237-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample Key=msrp_display_actual_price_type, value=Use config Key=gift_message_available, value=No Key=size, value=100 μg/vial Key=protein_name, value=Hemoglobin subunit alpha Key=gene_full_name, value=Hemoglobin subunit alpha Key=uniprot_id, value=Q8R2E7 Key=reconstitution, value=Add 0.2ml of distilled water will yield a concentration of 500μg/ml. Key=applications, value=IHC, WB Key=reactivity, value=Mouse, Rat Key=form, value=Lyophilized Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. Key=cross_reactivity, value=No cross-reactivity with other proteins Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). Key=predicted_reactivity Key=source_company, value=Boster A00237-4 Key=isotype, value=Rabbit IgG Key=molecular_weight, value=15258 MW Key=sample_size_available, value=30ug for $99, contact us for details Key=related_product_skus, value=B0001-5,AR1156,AR0146 Key=product_category, value=Primary Antibodies Key=research_category, value=Adaptive Immunity, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology Key=clonality, value=Polyclonal Key=swatch_image, value=/a/0/a00237-4-fadd-primary-antibodies-wb-testing-1.jpg Key=rating_value, value=100 Key=review_count, value=5 Key=phospho_site Key=mp_exclude_sitemap, value=No Key=yoast_facebook_image, value=/a/0/a00233-2-hab1-hab2-primary-antibodies-wb-testing-1.jpg Key=yoast_twitter_image, value=/a/0/a00233-2-hab1-hab2-primary-antibodies-wb-testing-1.jpg Key=cj_related_products, value=Apoptotic Process:PA1975|A02152^Necroptosis:PA2242|A01403-1^Release Of Cytochrome C From Mitochondria:PROTQ64373 Key=description, value=Boster Bio Anti-FADD Antibody Picoband® catalog # A00237-4. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Key=short_description, value=Boster Bio Anti-FADD Antibody Picoband® catalog # A00237-4. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Key=meta_keyword, value=Anti-FADD Antibody Picoband® Key=contents, value=Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3. Key=background, value=FADD, Fas-Associated protein with Death Domain, is a universal adaptor protein in apoptosis that mediates signaling of all known death domain-containing members of the TNF receptor superfamily. The FADD gene contains 2 exons and spans approximately 3.6 kb. By analysis of somatic cell hybrid panels and by fluorescence in situ hybridization, the FADD gene is mapped to 11q13.3. The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, thus, it participates in the death signaling initiated by these receptors. Key=gene_name, value=Fadd Key=synonyms, value=Hemoglobin subunit alpha;Alpha-globin;Hemoglobin alpha chain;HBA1;HBA2; Key=application_details, value=Western blot, 0.1-0.25μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Rat
Key=subcellular_localization, value= Secreted. Key=tissue_specificity, value= Red blood cells. Key=protein_function, value= Involved in oxygen transport from the lung to the various peripheral tissues. Key=application_notes, value=Tested Species: In-house tested species with positive results.
Other applications have not been tested.
Optimal dilutions should be determined by end users. Key=competitor_equivalent_skus, value=sc 31915|sc 48166|sc 20357|sc 20356|sc 48167|sc 166574|sc 166545|sc 137179|sc 20358|sc 32233|sc 59540|sc 47724|sc 365062|sc 25778|sc 66163|sc 81545|sc 69778|sc 51907|sc 51905|sc 20357 R Key=immunogen, value=A synthetic peptide corresponding to a sequence at the C-terminus of rat FADD, which shares 66.7% and 81.8% amino acid (aa) sequence identity with human and rat FADD, respectively. Key=purification, value=Immunogen affinity purified. Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. Key=custom_attribute_1, value=30 kDa Key=custom_attribute_2, value=WB: rat brain tissue, rat thymus tissue, rat PC-12 whole cell, mouse brain tissue, mouse thymus tissue, mouse NIH/3T3 whole cell IHC: rat liver tissue Key=options Key=media_gallery Key=extension_attributes Key=tier_price Key=tier_price_changed Key=category_ids Key=is_salable Key=website_ids Key=request_path Key=rating_summary Key=_cache_instance_store_filter Key=media_gallery_images
Anti-FADD Antibody Picoband®
FADD antibody
Boster Bio Anti-FADD Antibody Picoband® catalog # A00237-4. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Boster Bio Anti-FADD Antibody Picoband® catalog # A00237-4. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FADD Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00237-4)
A synthetic peptide corresponding to a sequence at the C-terminus of rat FADD, which shares 66.7% and 81.8% amino acid (aa) sequence identity with human and rat FADD, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A00237-4 is reactive to Fadd in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
30 kDa
Calculated molecular weight
15258 MW
Background of FADD
FADD, Fas-Associated protein with Death Domain, is a universal adaptor protein in apoptosis that mediates signaling of all known death domain-containing members of the TNF receptor superfamily. The FADD gene contains 2 exons and spans approximately 3.6 kb. By analysis of somatic cell hybrid panels and by fluorescence in situ hybridization, the FADD gene is mapped to 11q13.3. The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, thus, it participates in the death signaling initiated by these receptors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Rat
Positive Control
WB: rat brain tissue, rat thymus tissue, rat PC-12 whole cell, mouse brain tissue, mouse thymus tissue, mouse NIH/3T3 whole cell IHC: rat liver tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of FADD using anti-FADD antibody (A00237-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse thymus tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FADD antigen affinity purified polyclonal antibody (Catalog # A00237-4) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FADD at approximately 30KD. The expected band size for FADD is at 23KD.
Click image to see more details
Figure 2. IHC analysis of FADD using anti-FADD antibody (A00237-4).
FADD was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FADD Antibody (A00237-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Gene/Protein Information For Fadd (Source: Uniprot.org, NCBI)
Gene Name
Fadd
Full Name
FAS-associated death domain protein
Weight
15258 MW
Alternative Names
Hemoglobin subunit alpha;Alpha-globin;Hemoglobin alpha chain;HBA1;HBA2; FADD GIG3, MORT1 Fas associated via death domain FAS-associated death domain protein|Fas (TNFRSF6)-associated via death domain|Fas-associating death domain-containing protein|Fas-associating protein with death domain|growth-inhibiting gene 3 protein|mediator of receptor-induced toxicity
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on Fadd, check out the Fadd Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Fadd: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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