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Product Info Summary
| SKU: | A04664 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-Factor XIII B F13B Antibody
View all Factor XIIIb Antibodies
SKU/Catalog Number
A04664
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Factor XIII B F13B Antibody catalog # A04664. Tested in IHC-P, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Factor XIII B F13B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04664)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human F13B. AA range:500-550
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04664 is reactive to F13B in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
75511 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04664 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IHC-P, 1:100-1:300
Validation Images & Assay Conditions
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Western Blot (WB) analysis of Mouse Lung Mouse Kidney Mouse Heart Mouse Liver lysis using F13B antibody.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Liver, antibody was diluted at 1:200.
Protein Target Info & Infographic
Gene/Protein Information For F13B (Source: Uniprot.org, NCBI)
Gene Name
F13B
Full Name
Coagulation factor XIII B chain
Weight
75511 MW
Alternative Names
Coagulation factor XIII B chain;Fibrin-stabilizing factor B subunit;Protein-glutamine gamma-glutamyltransferase B chain;Transglutaminase B chain;F13B; F13B FXIIIB coagulation factor XIII B chain coagulation factor XIII B chain|TGase|coagulation factor XIII, B polypeptide|fibrin-stabilizing factor B subunit|protein-glutamine gamma-glutamyltransferase B chain|transglutaminase B chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on F13B, check out the F13B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for F13B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Factor XIII B F13B Antibody (A04664)
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No publications found for A04664
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-Factor XIII B F13B Antibody
Question
Is a blocking peptide available for product anti-Factor XIII B antibody (A04664)?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
We do provide the blocking peptide for product anti-Factor XIII B antibody (A04664). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-16
Question
Will anti-Factor XIII B antibody A04664 work for WB with liver?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
According to the expression profile of liver, F13B is highly expressed in liver. So, it is likely that anti-Factor XIII B antibody A04664 will work for WB with liver.
Boster Scientific Support
Answered: 2020-01-06
Question
Would A04664 anti-Factor XIII B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
It shows on the product datasheet, A04664 anti-Factor XIII B antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-16
Question
I see that the anti-Factor XIII B antibody A04664 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-20
Question
See attached the WB image, lot number and protocol we used for liver using anti-Factor XIII B antibody A04664. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-14
Question
Would anti-Factor XIII B antibody A04664 work on dog IHC with heart?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
Our lab technicians have not tested anti-Factor XIII B antibody A04664 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Factor XIII B antibody A04664 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog heart in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-22
Question
Do you have a BSA free version of anti-Factor XIII B antibody A04664 available?
Verified Customer
Verified customer
Asked: 2018-08-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Factor XIII B antibody A04664 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-08-24
Question
Can you help my question with product A04664, anti-Factor XIII B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-03-23
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04664 anti-Factor XIII B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-03-23
Question
I was wanting to use your anti-Factor XIII B antibody for WB for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
N. Johnson
Verified customer
Asked: 2017-09-28
Answer
It shows on the product datasheet, A04664 anti-Factor XIII B antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-28
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Factor XIII B antibody A04664. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-05-15
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-15
Question
Is this A04664 anti-Factor XIII B antibody reactive to the isotypes of F13B?
M. Lewis
Verified customer
Asked: 2015-06-26
Answer
The immunogen of A04664 anti-Factor XIII B antibody is Synthesized peptide derived from the Internal region of human Factor XIII B. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-06-26
Question
We are currently using anti-Factor XIII B antibody A04664 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
P. Evans
Verified customer
Asked: 2014-12-04
Answer
The anti-Factor XIII B antibody (A04664) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-12-04
Question
I am looking for to test anti-Factor XIII B antibody A04664 on human liver for research purposes, then I may be interested in using anti-Factor XIII B antibody A04664 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
G. Carter
Verified customer
Asked: 2014-05-14
Answer
The products we sell, including anti-Factor XIII B antibody A04664, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-05-14
