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Product Info Summary
| SKU: | M02047 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC |
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Product info
Product Name
Anti-Factor XIIIa (Coagulation Factor XIIIA Chain) Monoclonal Antibody
View all Factor XIIIa Antibodies
SKU/Catalog Number
M02047
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Factor XIIIa (Coagulation Factor XIIIA Chain) Monoclonal Antibody (Catalog # M02047). Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Factor XIIIa (Coagulation Factor XIIIA Chain) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02047)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: F13A1/1683
Isotype
IgG2b, kappa
Immunogen
Recombinant fragment of human Factor XIIIa protein (aa46-181) (exact sequence is proprietary)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M02047 is reactive to F13A1 in Human
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
83267 MW
Background of Factor XIIIa
The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. This monoclonal antibody recognizes a protein of 83kDa, which is identified as Factor XIIIa. It has been identified in platelets, megakaryocytes, and fibroblast-like mesenchymal or histiocytic cells in the placenta, uterus, and prostate, monocytes and macrophages and dermal dendritic cells. Anti-factor XIIIa has been found to be useful in differentiating between dermatofibroma (almost all cases are positive), dermatofibrosarcoma protuberans (-/+) and desmoplastic malignant melanoma (-). Anti-factor XIIIa positivity is also seen in capillary hemagioblastoma, hemangioendothelioma, hemangiopericytoma, xanthogranuloma, xanthoma, hepatocellular carcinoma, glomus tumor, and meningioma.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02047 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Histiocytoma stained with Anti-Factor XIIIa Mouse Monoclonal Antibody (F13A1/1683).
Protein Target Info & Infographic
Gene/Protein Information For F13A1 (Source: Uniprot.org, NCBI)
Gene Name
F13A1
Full Name
Coagulation factor XIII A chain
Weight
83267 MW
Superfamily
transglutaminase superfamily
Alternative Names
Coagulation factor XIII A chain;Coagulation factor XIIIa;2.3.2.13;Protein-glutamine gamma-glutamyltransferase A chain;Transglutaminase A chain;F13A1;F13A; F13A1 F13A coagulation factor XIII A chain coagulation factor XIII A chain|FSF, A subunit|TGase|bA525O21.1 (coagulation factor XIII, A1 polypeptide)|coagulation factor XIII, A polypeptide|coagulation factor XIII, A1 polypeptide|coagulation factor XIIIa|factor XIIIa|fibrin stabilizing factor, A subunit|fibrinoligase|protein-glutamine gamma-glutamyltransferase A chain|transglutaminase A chain|transglutaminase. plasma
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on F13A1, check out the F13A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for F13A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Factor XIIIa (Coagulation Factor XIIIA Chain) Monoclonal Antibody (M02047)
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No publications found for M02047
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Customer Q&As
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6 Customer Q&As for Anti-Factor XIIIa (Coagulation Factor XIIIA Chain) Monoclonal Antibody
Question
My lab would like to test anti-Factor XIIIa (Coagulation Factor XIIIA Chain) Monoclonal antibody M02047 on human esophagus for research purposes, then I may be interested in using anti-Factor XIIIa (Coagulation Factor XIIIA Chain) Monoclonal antibody M02047 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
The products we sell, including anti-Factor XIIIa (Coagulation Factor XIIIA Chain) Monoclonal antibody M02047, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-18
Question
Will anti-Factor XIIIa (Coagulation Factor XIIIA Chain) Monoclonal antibody M02047 work on feline IHC with pancreas?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
Our lab technicians have not validated anti-Factor XIIIa (Coagulation Factor XIIIA Chain) Monoclonal antibody M02047 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Factor XIIIa (Coagulation Factor XIIIA Chain) Monoclonal antibody M02047 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline pancreas in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-03
Question
I have a question about product M02047, anti-Factor XIIIa (Coagulation Factor XIIIA Chain) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02047 anti-Factor XIIIa (Coagulation Factor XIIIA Chain) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-02
Question
I see that the anti-Factor XIIIa (Coagulation Factor XIIIA Chain) Monoclonal antibody M02047 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-28
Question
I was wanting to use your anti-Factor XIIIa (Coagulation Factor XIIIA Chain) Monoclonal antibody for IHC for human esophagus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human esophagus identification?
Verified Customer
Verified customer
Asked: 2017-06-20
Answer
It shows on the product datasheet, M02047 anti-Factor XIIIa (Coagulation Factor XIIIA Chain) Monoclonal antibody has been validated for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human esophagus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-20
Question
Is a blocking peptide available for product anti-Factor XIIIa (Coagulation Factor XIIIA Chain) Monoclonal antibody (M02047)?
S. Collins
Verified customer
Asked: 2013-01-14
Answer
We do provide the blocking peptide for product anti-Factor XIIIa (Coagulation Factor XIIIA Chain) Monoclonal antibody (M02047). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-01-14
