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Product Info Summary
| SKU: | PB9934 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Factor B/CFB Antibody Picoband®
View all Complement Factor B Antibodies
SKU/Catalog Number
PB9934
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Factor B/CFB Antibody Picoband® catalog # PB9934. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Factor B/CFB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9934)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Factor B recombinant protein (Position: E518-L764). Human Factor B shares 84.2% amino acid (aa) sequence identity with mouse Factor B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9934 is reactive to CFB in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
86 kDa
Calculated molecular weight
85533 MW
Background of Complement Factor B
Complement factor B is a protein that in humans is encoded by the CFB gene. This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9934 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: A549 whole cell, 293T whole cell, HELA whole cell
IHC: human lung cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Factor B using anti-Factor B antibody (PB9934).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: A549 whole cell lysates,
Lane 2: 293T whole cell lysates,
Lane 3: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor B antigen affinity purified polyclonal antibody (Catalog # PB9934) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Factor B at approximately 86 kDa. The expected band size for Factor B is at 86 kDa.
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Figure 2. IHC analysis of Factor B using anti-Factor B antibody (PB9934).
Factor B was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Factor B Antibody (PB9934) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CFB (Source: Uniprot.org, NCBI)
Gene Name
CFB
Full Name
Complement factor B
Weight
85533 MW
Superfamily
peptidase S1 family
Alternative Names
Complement factor B;3.4.21.47;C3/C5 convertase;Glycine-rich beta glycoprotein;GBG;PBF2;Properdin factor B;Complement factor B Ba fragment;Complement factor B Bb fragment;CFB;BF, BFD; CFB AHUS4, ARMD14, BF, BFD, CFABD, FB, FBI12, GBG, H2-Bf, PBF2, CFB complement factor B complement factor B|B-factor, properdin|C3 proaccelerator|C3 proactivator|C3/C5 convertase|glycine-rich beta-glycoprotein|properdin factor B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CFB, check out the CFB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CFB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Factor B/CFB Antibody Picoband® (PB9934)
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No publications found for PB9934
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Customer Q&As
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3 Customer Q&As for Anti-Factor B/CFB Antibody Picoband®
Question
Is this PB9934 anti-Factor B/CFB antibody reactive to the isotypes of CFB?
G. Zhao
Verified customer
Asked: 2020-03-27
Answer
The immunogen of PB9934 anti-Factor B/CFB antibody is E. coli-derived human Factor B recombinant protein (Position: E518-L764). Human Factor B shares 84.2% amino acid (aa) sequence identity with mouse Factor B. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-27
Question
See below the WB image, lot number and protocol we used for right lobe of liver using anti-Factor B/CFB antibody PB9934. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-05
Question
We are currently using anti-Factor B/CFB antibody PB9934 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-09
Answer
The anti-Factor B/CFB antibody (PB9934) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-09
