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Product Info Summary
| SKU: | RP1085 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-FABP4 Antibody Picoband®
View all FABP4/A-FABP Antibodies
SKU/Catalog Number
RP1085
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FABP4 Antibody catalog # RP1085. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FABP4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1085)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human FABP4, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1085 is reactive to FABP4 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
15 kDa
Calculated molecular weight
14719 MW
Background of FABP4/A-FABP
Fatty acid binding proteins (FABPs) are small cytoplasmic proteins that are expressed in a highly tissue-specific manner and bind to fatty acids such as oleic and retinoic acid. Adipocyte fatty-acid-binding protein, aP2 (FABP4) is expressed in adipocytes and macrophages, and integrates inflammatory and metabolic responses. Studies in aP2-deficient mice have shown that this lipid chaperone has a significant role in several aspects of metabolic syndrome, including type 2 diabetes and atherosclerosis. It regulates allergic airway inflammation and may provide a link between fatty acid metabolism and asthma.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1085 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human RT4 whole cell, human U251 whole cell, rat heart tissue, mouse heart tissue
IHC: Mouse Intestine tissue, Rat Intestine tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FABP4 using anti-FABP4 antibody (RP1085).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: rat heart tissue lysates,
Lane 4: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP4 antigen affinity purified polyclonal antibody (Catalog # RP1085) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FABP4 at approximately 15 kDa. The expected band size for FABP4 is at 15 kDa.
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Figure 2. IHC analysis of FABP4 using anti-FABP4 antibody (RP1085).
FABP4 was detected in a paraffin-embedded section of Mouse Intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-FABP4 Antibody (RP1085) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis of FABP4 using anti-FABP4 antibody (RP1085).
FABP4 was detected in a paraffin-embedded section of Rat Intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-FABP4 Antibody (RP1085) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For FABP4 (Source: Uniprot.org, NCBI)
Gene Name
FABP4
Full Name
Fatty acid-binding protein, adipocyte
Weight
14719 MW
Superfamily
calycin superfamily
Alternative Names
Fatty acid-binding protein, adipocyte;Adipocyte lipid-binding protein;ALBP;Adipocyte-type fatty acid-binding protein;A-FABP;AFABP;Fatty acid-binding protein 4;FABP4; FABP4 A-FABP, AFABP, ALBP, HEL-S-104, aP2 fatty acid binding protein 4 fatty acid-binding protein, adipocyte|adipocyte fatty acid binding protein|adipocyte lipid-binding protein|adipocyte-type fatty acid-binding protein|epididymis secretory protein Li 104|fatty acid binding protein 4, adipocyte
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FABP4, check out the FABP4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FABP4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FABP4 Antibody Picoband® (RP1085)
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Customer Q&As
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5 Customer Q&As for Anti-FABP4 Antibody Picoband®
Question
What is the method of purification for RP1085?
Verified customer
Asked: 2019-09-13
Answer
For the Anti-FABP4 Antibody (RP1085), the purification method is Immunogen affinity purified.
Boster Scientific Support
Answered: 2019-09-13
Question
We are currently using anti-FABP4 antibody RP1085 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
The anti-FABP4 antibody (RP1085) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-11
Question
Would anti-FABP4 antibody RP1085 work on primate IHC with liver?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
Our lab technicians have not tested anti-FABP4 antibody RP1085 on primate. You can run a BLAST between primate and the immunogen sequence of anti-FABP4 antibody RP1085 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate liver in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-12
Question
Will anti-FABP4 antibody RP1085 work for WB with urinary bladder?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
According to the expression profile of urinary bladder, FABP4 is highly expressed in urinary bladder. So, it is likely that anti-FABP4 antibody RP1085 will work for WB with urinary bladder.
Boster Scientific Support
Answered: 2019-06-17
Question
Is a blocking peptide available for product anti-FABP4 antibody (RP1085)?
Verified Customer
Verified customer
Asked: 2017-08-21
Answer
We do provide the blocking peptide for product anti-FABP4 antibody (RP1085). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-08-21
