Anti-F2 Antibody (N-Term)

Product Name

Anti-F2 Antibody (N-Term)

View all Coagulation Factor II/Thrombin Antibodies

SKU/Catalog Number

M00044-1

Size

50 µl

Description

Boster Bio Anti-F2 Antibody (N-Term) (Catalog # M00044-1). Tested in WB application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-F2 Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00044-1)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This F2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 46-79 amino acids from human F2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00044-1 is reactive to F2 in Human

Applications

M00044-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

70037 Da

Background of Coagulation Factor II/Thrombin

Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:2000

Validation Images & Assay Conditions

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All lanes : Anti-F2 Antibody (N-Term) at 1:2000 dilution
Lane 1: HL-60 whole cell lysates
Lane 2: A549 whole cell lysates
Lane 3: human plasma lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 70 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Gene/Protein Information For F2 (Source: Uniprot.org, NCBI)

Alternative Names

coagulation factor II (thrombin) receptor-like 2; Coagulation factor II receptor-like 2 (protease-actovated receptor 3); Coagulation factor II receptor-like 2; Coagulation Factor II; F2; PAR-3; PAR3proteinase-activated receptor 3; protease-activated receptor 3; proteinase-activated receptor-3; PT; serine protease; Thrombin receptor-like 2; Thrombin F2 PT, RPRGL2, THPH1 coagulation factor II, thrombin prothrombin|prepro-coagulation factor II|prothrombin B-chain|thrombin factor II

For more info on F2, check out the F2 Infographic

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In this infographic, you will see the following information for F2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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