Product Info Summary
SKU: | A02900-3 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Exportin-5/XPO5 Antibody Picoband®
View all Exportin-5 Antibodies
SKU/Catalog Number
A02900-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Exportin-5/XPO5 Antibody Picoband® catalog # A02900-3. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Exportin-5/XPO5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02900-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Exportin-5/XPO5 recombinant protein (Position: M1-L1104).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02900-3 is reactive to XPO5 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
130 kDa
Calculated molecular weight
136311 MW
Background of Exportin-5
Exportin-5 (XPO5) is a protein that in humans is encoded by the XPO5 gene. The International Radiation Hybrid Mapping Consortium mapped the XPO5 gene to chromosome 6. This gene encodes a member of the karyopherin family that is required for the transport of small RNAs and double-stranded RNA-binding proteins from the nucleus to the cytoplasm. The encoded protein translocates cargo through the nuclear pore complex in a RanGTP-dependent process.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02900-3 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Positive Control
WB: human CACO-2 whole cell, human Daudi whole cell
ICC/IF: U20S cell
FCM: Daudi cell
Validation Images & Assay Conditions
![a02900 3 xpo5 primary antibodies wb testing 1 a02900 3 xpo5 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a02900-3-xpo5-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of Exportin-5/XPO5 using anti-Exportin-5/XPO5 antibody (A02900-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human CACO-2 whole cell lysates,
Lane 2: human Daudi whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Exportin-5/XPO5 antigen affinity purified polyclonal antibody (Catalog # A02900-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Exportin-5/XPO5 at approximately 130 kDa. The expected band size for Exportin-5/XPO5 is at 136 kDa.
![a02900 3 xpo5 primary antibodies if testing 2 a02900 3 xpo5 primary antibodies if testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a02900-3-xpo5-primary-antibodies-if-testing-2.jpg)
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Figure 2. IF analysis of Exportin-5/XPO5 using anti-Exportin-5/XPO5 antibody (A02900-3).
Exportin-5/XPO5 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Exportin-5/XPO5 Antibody (A02900-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a02900 3 xpo5 primary antibodies fcm testing 3 a02900 3 xpo5 primary antibodies fcm testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a02900-3-xpo5-primary-antibodies-fcm-testing-3.jpg)
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Figure 3. Flow Cytometry analysis of Daudi cells using anti-Exportin-5/XPO5 antibody (A02900-3).
Overlay histogram showing Daudi cells stained with A02900-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Exportin-5/XPO5 Antibody (A02900-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For XPO5 (Source: Uniprot.org, NCBI)
Gene Name
XPO5
Full Name
Exportin-5
Weight
136311 MW
Superfamily
exportin family
Alternative Names
Exportin-5;Exp5;Ran-binding protein 21;XPO5;KIAA1291, RANBP21; XPO5 exp5 exportin 5 exportin-5|ran-binding protein 21
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on XPO5, check out the XPO5 Infographic
![XPO5 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for XPO5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Exportin-5/XPO5 Antibody Picoband® (A02900-3)
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