Exportin-5 Antibodies

Exportin-5 (XPO5)

XPO5 in the nucleus binds cooperatively to the RNA and to the GTPase Ran in its busy GTP-bound form. Proteins containing dsRBDs can associate with this trimeric complex through the RNA.

Docking of the complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP into Ran-GDP (triggered by RANBP1 and RANGAP1, respectively) trigger disassembly of the complex and discharge of the cargo from the export receptor.

Gene Information

Human
Gene Name:	XPO5
Uniprot:	Q9HAV4
Entrez:	57510

Family

Belongs to:
exportin family

Alternative Names

Exp5; exportin 5; exportin-5; FLJ14239; FLJ32057; FLJ45606; KIAA1291exp5; Ran-binding protein 21; RANBP21

Molecular Weight

Mass (kDA):
136.311 kDA

Genomic Context

Human
Location:	6p21.1
Sequence:	6; NC_000006.12 (43522330..43576075, complement)

Tissue Specificity

Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas.

Subcellular Localizations

Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm.

Diseases

• Malignant Neoplasms
• Carcinoma
• Neoplasms
• Microsatellite Instability
• Malignant Neoplasm Of Lung
• Carcinogenesis
• Colorectal Cancer
• Cell Transformation, Neoplastic
• Lung Neoplasms
• Mutation, Out-of-frame
• Hepatitis B
• Hepatitis
• Malignant Neoplasm Of Ovary

• Malignant Squamous Cell Neoplasm
• Malignant Neoplasm Of Stomach
• Cholangiocarcinoma
• Stomach Neoplasms
• Neoplasms, Second Primary
• Malignant Neoplasm Of Breast
• Kidney Neoplasm

Pathways

• Nuclear Export
• Rna Interference
• Transport
• Localization
• Gene Silencing
• Nuclear Transport
• Translation
• Nuclear Import
• Mismatch Repair
• Nucleocytoplasmic Transport
• Myoblast Proliferation
• Skeletal Muscle Hypertrophy
• Regulation Of Gene Expression

• Ribosome Assembly
• Pre-mirna Processing
• Oncogenesis
• Vegetative Phase Change
• Dna Repair
• Rna Localization
• Methylation

PTMs

• Methylation

• Phosphorylation

Research Areas

• Epigenetics

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/XPO5

Boster Bio Antibodies - Best Uses Of The XPO5 Marker

If you're looking for a new antibody for exportin-5, you've come to the right place! Boster Bio has been producing high affinity primary antibodies for the last 25 years, and these are highly cited and trusted by the research community. Boster antibodies have also been validated using Western Blotting, ELISA, Immunohistochemistry, and Western Blotting. Learn more about these antibodies and their best uses!

Exportin-5 antibodies

The XPO5 genetic gene is one the most frequently expressed on eukaryotic cellular cells. This makes it a promising target to study cancer. It has been associated with numerous cancers, including gastric cancer. It is used for cancer diagnosis, research on gastrointestinal stromal cells, as well as cancer immunotherapy. XPO5 is useful for cell viability analysis and proliferation.

IHC kit (Boster).

Steven Xia was the famed histologist who founded Boster Bio. The company specializes in producing high-affinity and high-specificity antibodies, ELISA kits, and other related products. Boster Bio products have been cited in more than 18,000 publications, and are transparently validated for many applications, including Flow Cytometry. Boster Bio is committed in customer service and quality and offers a 100% quality guarantee on all products. They also continue to expand their product lines each month.

Enhanced Chemiluminescence Identification (ECL).

Enhanced Chemiluminescence is a technique that uses antibodies that are conjugated to HP. It is one of most popular methods to measure the protein concentrations within cellular extracts. This reaction is when the enzyme interacts to a chemiluminescent medium, which creates excited intermediates that emit strong, blue light at 450nm. This light emission occurs only during the enzyme-substrate interaction and ends when the substrate is depleted.

To perform the analysis, you must prepare the membrane as described above. Prepare a lysate for one cell from the adult testis. This is made by mixing 50mM Tris, pH 7.4 with 11% NP-40 (v/v), and protease/phosphatase inhibit cocktails. The protein assay kit Pierce BCA (Thermo scientific) was used to measure the proteins using SDS-PAGE under reducing conditions. Next, transfer your protein-bound sample onto a nitrocellulose cell membrane.

High Sensitivity ECLSubstrate Kit allows for protein detection in bands between 23pg and 182ng. This kit uses a specially developed enhanced chemiluminescent substrat for this purpose. It has been optimized for both film and CCD imaging. ECL reagents with high sensitivity provide bright signals and low background. This allows you to take multiple exposures without losing any valuable data.

The XPO5 chemiluminescent chemiluminescent material can be used to detect the protein concentrations in a western blot experiment. These substrates can be used with both chromatin-stained and protein abundance membranes. The SuperSignal West PicoPlus Chemiluminescent substrate works well in western blot applications containing abundant proteins. The SuperSignal West Pico Plus Chemiluminescent substrate has been developed for sensitive and high-specific protein detection.

Colorimetric detection

The need for a rapid test to detect V.parahaemolyticus motivated the development a colorimetric detector protocol. A specific sequence Vp was created, integrated with an forward primer, and then treated as an HPR-mimicking DNAzyme. This DNAzyme is amplified in the polymerase chains reaction (PCR). The products from PCR with the HRP mimicking DNAzyme displayed distinct colors when they were combined with catalysis substrats.

Colorimetric Western blotting has a high level of sensitive detection. The detection method of colorimetric Western blotting is highly sensitive, but prolonged incubation can lead to an increase in background signal, which can obscure or mask the signal of the protein. Another drawback of this technique is its inability to be used for proteins with low abundance. The method is inexpensive and easy to perform, despite its limitations. Moreover, it is available in many colorimetric substrates, which produce different colored precipitates and offer different sensitivity.

The method is extremely sensitive and easy to implement. This method can be used for routine screenings in areas with limited resources. The sensitivity and quantitative detection capabilities of the method can be improved with a new version. You can use it for on site detection in the future. It is expected that it will be a standard test to detect on-site in a variety field situations.

Two colorimetric methods can be used for detecting methylated DNA. The HRP/H2O2 system uses the presence of chromogen, to produce a coloured side-product. The XPO5 indicator is used for the other. The H2O2 system is a popular colorimetric detection method. The HRP/H2O2 method combines peroxidase and chromogen enzymes to produce a coloured byproduct.