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Product Info Summary
| SKU: | A00536 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-Exonuclease 1 Exo1 Antibody
View all Exonuclease 1 Antibodies
SKU/Catalog Number
A00536
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Exonuclease 1 Exo1 Antibody catalog # A00536. Tested in ELISA, IF, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Exonuclease 1 Exo1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00536)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human EXO1. AA range:61-110
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00536 is reactive to EXO1 in Human, Mouse
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
94103 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00536 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Western blot analysis of various cells using Exo1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
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Immunofluorescence analysis of A549 cells, using EXO1 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For EXO1 (Source: Uniprot.org, NCBI)
Gene Name
EXO1
Full Name
Exonuclease 1
Weight
94103 MW
Superfamily
XPG/RAD2 endonuclease family
Alternative Names
Exonuclease 1;hExo1;3.1.-.-;Exonuclease I;hExoI;EXO1;EXOI, HEX1; EXO1 HEX1, hExoI exonuclease 1 exonuclease 1|rad2 nuclease family member, homolog of S. cerevisiae exonuclease 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EXO1, check out the EXO1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EXO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Exonuclease 1 Exo1 Antibody (A00536)
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No publications found for A00536
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Exonuclease 1 Exo1 Antibody
Question
Is this A00536 anti-Exo1 antibody reactive to the isotypes of EXO1?
R. Parker
Verified customer
Asked: 2020-03-19
Answer
The immunogen of A00536 anti-Exo1 antibody is Synthesized peptide derived from the N-terminal region of human Exo1. at AA rangle: 30-110. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-19
Question
I see that the anti-Exo1 antibody A00536 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-23
Question
Do you have a BSA free version of anti-Exo1 antibody A00536 available?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Exo1 antibody A00536 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-13
Question
Will anti-Exo1 antibody A00536 work on goat WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
Our lab technicians have not tested anti-Exo1 antibody A00536 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Exo1 antibody A00536 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-14
Question
See below the WB image, lot number and protocol we used for lung using anti-Exo1 antibody A00536. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-29
Question
I would like to test anti-Exo1 antibody A00536 on mouse lung for research purposes, then I may be interested in using anti-Exo1 antibody A00536 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-11-24
Answer
The products we sell, including anti-Exo1 antibody A00536, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-11-24
Question
Would A00536 anti-Exo1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Wu
Verified customer
Asked: 2015-11-10
Answer
As indicated on the product datasheet, A00536 anti-Exo1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-11-10
