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Product Info Summary
| SKU: | PA1589-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-GRB7 Antibody Picoband®
SKU/Catalog Number
PA1589-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GRB7 Antibody catalog # PA1589-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GRB7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1589-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human GRB7, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1589-1 is reactive to GRB7 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
59681 MW
Background of GRB7
GRB7, growth factor receptor-bound protein 7, is a protein which in humans is encoded by the GRB7 gene. The product of this gene belongs to a small family of adaptor proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. The protein plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Alternative splicing results in multiple transcript variants encoding different isoforms, although the full-length natures of only two of the variants have been determined to date. GRB7 is an SH2-domain adaptor protein that binds to receptor tyrosine kinases and provides the intra-cellular direct link to the Ras proto-oncogene. Human GRB7 is located on the long arm of chromosome 17, next to the ERBB2 (alias HER2/neu) proto-oncogene. These two genes are commonly co-amplified (present in excess copies) in breast cancers. GRB7 thought to be involved in migration, is well known to be over-expressed in testicular germ cell tumors, esophageal cancers, and gastric cancers.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1589-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Testis Tissue, SMMC Whole Cell, HELA Whole Cell, A549 Whole Cell, SW620 Whole Cell
IHC: Human Oesophagus Squama Cancer Tissue
Validation Images & Assay Conditions
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Anti-GRB7 antibody, PA1589-1, IHC(P)
IHC(P): Human Oesophagus Squama Cancer Tissue
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Anti-GRB7 antibody, PA1589-1, All Western blotting
All lanes: Anti-GRB7(PA1589-1) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: A549 Whole Cell Lysate at 40ug
Lane 5: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 60KD
Observed bind size: 60KD
Protein Target Info & Infographic
Gene/Protein Information For GRB7 (Source: Uniprot.org, NCBI)
Gene Name
GRB7
Full Name
Growth factor receptor-bound protein 7
Weight
59681 MW
Superfamily
GRB7/10/14 family
Alternative Names
Growth factor receptor-bound protein 7;B47;Epidermal growth factor receptor GRB-7;GRB7 adapter protein;GRB7; GRB7 growth factor receptor bound protein 7 growth factor receptor-bound protein 7|B47|GRB7 adapter protein|epidermal growth factor receptor GRB-7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GRB7, check out the GRB7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GRB7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GRB7 Antibody Picoband® (PA1589-1)
Hello CJ!
PA1589-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Grb7 gene amplification and protein expression by FISH and IHC in ovarian cancer
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-GRB7 Antibody Picoband®
Question
Does anti-GRB7 antibody PA1589-1 work on monkey IHC with prostate thalamus?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
Our lab technicians have not validated anti-GRB7 antibody PA1589-1 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-GRB7 antibody PA1589-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey prostate thalamus in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-20
Question
We are currently using anti-GRB7 antibody PA1589-1 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
The anti-GRB7 antibody (PA1589-1) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-08
Question
Is a blocking peptide available for product anti-GRB7 antibody (PA1589-1)?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
We do provide the blocking peptide for product anti-GRB7 antibody (PA1589-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-29
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-GRB7 antibody PA1589-1. Let me know if you need anything else.
S. Jha
Verified customer
Asked: 2016-10-19
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-10-19
Question
Would anti-GRB7 antibody PA1589-1 work for WB with lung?
S. Mangal
Verified customer
Asked: 2013-11-01
Answer
According to the expression profile of lung, GRB7 is highly expressed in lung. So, it is likely that anti-GRB7 antibody PA1589-1 will work for WB with lung.
Boster Scientific Support
Answered: 2013-11-01
Question
I am interested in to test anti-GRB7 antibody PA1589-1 on mouse lung for research purposes, then I may be interested in using anti-GRB7 antibody PA1589-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
T. Zhao
Verified customer
Asked: 2013-03-19
Answer
The products we sell, including anti-GRB7 antibody PA1589-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-03-19
