Anti-ERK2 Monoclonal Antibody

Product Name

Anti-ERK2 Monoclonal Antibody

View all ERK2 Antibodies

SKU/Catalog Number

M00030

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-ERK2 Monoclonal Antibody catalog # M00030. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ERK2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00030)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

II-13

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human ERK2 Act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of ERK2 requires its phosphorylation by upstream kinases. ERK2 is located in the cytoplasm of resting cells and translocates into the nucleus upon extracellular stimuli by active transport of a dimer. ERK2 is essential for placental development and ERK2 in the trophoblast compartment may be indispensable for the vascularization of the labyrinth.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00030 is reactive to MAPK1 in Human, Mouse, Rat

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

131 kDa

Calculated molecular weight

88068 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00030 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:40
FC 1:40

Positive Control

IHC: Human melanoma tissue, Human renal cancer tissue, Rat lung tissue, Mouse ovary tissue, Rat skin tissue, human gastric adenocarcinoma tissue

Validation Images & Assay Conditions

Gene/Protein Information For MAPK1 (Source: Uniprot.org, NCBI)

Alternative Names

Signal transducer and activator of transcription 3;Acute-phase response factor;STAT3;APRF; MAPK1 ERK, ERK-2, ERK2, ERT1, MAPK2, NS13, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk, p42-MAPK mitogen-activated protein kinase 1 mitogen-activated protein kinase 1|MAP kinase 1|MAP kinase 2|MAPK 2|extracellular signal-regulated kinase 2|mitogen-activated protein kinase 2|protein tyrosine kinase ERK2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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