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Product Info Summary
| SKU: | A03411 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ERF Antibody
SKU/Catalog Number
A03411
Size
100μl
Form
Liquid
Description
Boster Bio Anti-ERF Antibody catalog # A03411. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ERF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03411)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human ERF around the non-phosphorylation site of T526.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03411 is reactive to ERF in Human, Mouse
Applications
A03411 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
58703 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of HeLa cells using ERF Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using ERF Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For ERF (Source: Uniprot.org, NCBI)
Gene Name
ERF
Full Name
ETS domain-containing transcription factor ERF
Weight
58703 MW
Superfamily
ETS family
Alternative Names
Ets2 repressor factorPE-2PE2ETS domain-containing transcription factor ERF ERF CHYTS, CRS4, PE-2, PE2 ETS2 repressor factor ETS domain-containing transcription factor ERF
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ERF, check out the ERF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ERF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ERF Antibody (A03411)
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No publications found for A03411
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-ERF Antibody
Question
Will anti-ERF antibody A03411 work on goat WB with left lobe of thyroid gland?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
Our lab technicians have not tested anti-ERF antibody A03411 on goat. You can run a BLAST between goat and the immunogen sequence of anti-ERF antibody A03411 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat left lobe of thyroid gland in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-20
Question
Is there a BSA free version of anti-ERF antibody A03411 available?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ERF antibody A03411 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-03
Question
Can you help my question with product A03411, anti-ERF antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03411 anti-ERF antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-17
Question
Is a blocking peptide available for product anti-ERF antibody (A03411)?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
We do provide the blocking peptide for product anti-ERF antibody (A03411). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-16
Question
We are currently using anti-ERF antibody A03411 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
The anti-ERF antibody (A03411) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-26
Question
See attached the WB image, lot number and protocol we used for liver using anti-ERF antibody A03411. Please let me know if you require anything else.
Z. Roberts
Verified customer
Asked: 2017-11-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-23
Question
Is this A03411 anti-ERF antibody reactive to the isotypes of ERF?
S. Lewis
Verified customer
Asked: 2016-04-07
Answer
The immunogen of A03411 anti-ERF antibody is Synthesized peptide derived from human ERF around the non-phosphorylation site of T526. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-04-07
