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Product Info Summary
| SKU: | M00539 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-ErbB (HER3) Rabbit Monoclonal Antibody
View all ErbB3/Her3 Antibodies
SKU/Catalog Number
M00539
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ErbB (HER3) Rabbit Monoclonal Antibody catalog # M00539. Tested in WB, IP applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ErbB (HER3) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00539)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BIB-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ErbB (HER3)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00539 is reactive to ERBB3 in Human, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
54 kDa
Calculated molecular weight
148098 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00539 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:30
Positive Control
WB: MCF-7 cell
Validation Images & Assay Conditions
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Western blot analysis of ErbB3 (HER3) expression in MCF-7 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For ERBB3 (Source: Uniprot.org, NCBI)
Gene Name
ERBB3
Full Name
Receptor tyrosine-protein kinase erbB-3
Weight
148098 MW
Superfamily
protein kinase superfamily
Alternative Names
Receptor tyrosine-protein kinase erbB-3;2.7.10.1;Proto-oncogene-like protein c-ErbB-3;Tyrosine kinase-type cell surface receptor HER3;ERBB3;HER3; ERBB3 ErbB-3, FERLK, HER3, LCCS2, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3 erb-b2 receptor tyrosine kinase 3 receptor tyrosine-protein kinase erbB-3|human epidermal growth factor receptor 3|proto-oncogene-like protein c-ErbB-3|tyrosine kinase-type cell surface receptor HER3|v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ERBB3, check out the ERBB3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ERBB3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ErbB (HER3) Rabbit Monoclonal Antibody (M00539)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-ErbB (HER3) Rabbit Monoclonal Antibody
Question
We purchased anti-ErbB (HER3) Rabbit Monoclonal antibody for WB on liver in the past. I am using human, and I plan to use the antibody for IP next. I was wanting to use examining liver as well as hippocampus in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
R. Li
Verified customer
Asked: 2020-03-11
Answer
I have checked the website and datasheets of our anti-ErbB (HER3) Rabbit Monoclonal antibody and it seems that M00539 has been validated on human in both WB and IP. Thus M00539 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-03-11
Question
I was wanting to use to test anti-ErbB (HER3) Rabbit Monoclonal antibody M00539 on rat placenta small intestine for research purposes, then I may be interested in using anti-ErbB (HER3) Rabbit Monoclonal antibody M00539 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
The products we sell, including anti-ErbB (HER3) Rabbit Monoclonal antibody M00539, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-10
Question
I was wanting to use your anti-ErbB (HER3) Rabbit Monoclonal antibody for WB for rat placenta small intestine on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat placenta small intestine identification?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
As indicated on the product datasheet, M00539 anti-ErbB (HER3) Rabbit Monoclonal antibody has been validated for IP, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat placenta small intestine in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-26
Question
Please see the WB image, lot number and protocol we used for placenta small intestine using anti-ErbB (HER3) Rabbit Monoclonal antibody M00539. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-24
Question
Our team were well pleased with the WB result of your anti-ErbB (HER3) Rabbit Monoclonal antibody. However we have observed positive staining in corpus callosum isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is ERBB3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
According to literature, corpus callosum does express ERBB3. Generally ERBB3 expresses in isoform 1: cell membrane. Regarding which tissues have ERBB3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Hippocampus, Placenta, and Small intestine, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 7685162
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-12-12
Question
Can you help my question with product M00539, anti-ErbB (HER3) Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00539 anti-ErbB (HER3) Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-03
Question
We have observed staining in rat placenta. Any tips? Is anti-ErbB (HER3) Rabbit Monoclonal antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
According to literature placenta does express ERBB3. According to Uniprot.org, ERBB3 is expressed in corpus callosum, placenta, hippocampus, placenta small intestine, skin, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have ERBB3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Hippocampus, Placenta, and Small intestine, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 7685162
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-10-10
Question
Is this M00539 anti-ErbB (HER3) Rabbit Monoclonal antibody reactive to the isotypes of ERBB3?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
The immunogen of M00539 anti-ErbB (HER3) Rabbit Monoclonal antibody is A synthesized peptide derived from human ErbB (HER3). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-24
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta small intestine using anti-ErbB (HER3) Rabbit Monoclonal antibody M00539. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-21
Question
We are currently using anti-ErbB (HER3) Rabbit Monoclonal antibody M00539 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-09
Answer
The anti-ErbB (HER3) Rabbit Monoclonal antibody (M00539) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-09
Question
Does anti-ErbB (HER3) Rabbit Monoclonal antibody M00539 work for WB with placenta small intestine?
E. Banerjee
Verified customer
Asked: 2018-01-03
Answer
According to the expression profile of placenta small intestine, ERBB3 is highly expressed in placenta small intestine. So, it is likely that anti-ErbB (HER3) Rabbit Monoclonal antibody M00539 will work for WB with placenta small intestine.
Boster Scientific Support
Answered: 2018-01-03
Question
Is a blocking peptide available for product anti-ErbB (HER3) Rabbit Monoclonal antibody (M00539)?
Verified Customer
Verified customer
Asked: 2017-09-13
Answer
We do provide the blocking peptide for product anti-ErbB (HER3) Rabbit Monoclonal antibody (M00539). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-09-13
Question
I see that the anti-ErbB (HER3) Rabbit Monoclonal antibody M00539 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-08-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-08-11
Question
Do you have a BSA free version of anti-ErbB (HER3) Rabbit Monoclonal antibody M00539 available?
N. Rodriguez
Verified customer
Asked: 2015-09-04
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-ErbB (HER3) Rabbit Monoclonal antibody M00539 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-09-04
Question
Would M00539 anti-ErbB (HER3) Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Krishna
Verified customer
Asked: 2013-09-06
Answer
As indicated on the product datasheet, M00539 anti-ErbB (HER3) Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-09-06
