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Product Info Summary
| SKU: | A00484-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Erythropoietin EPO Antibody Picoband®
View all Erythropoietin/EPO Antibodies
SKU/Catalog Number
A00484-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Erythropoietin EPO Antibody catalog # A00484-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Erythropoietin EPO Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00484-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human EPO recombinant protein (Position: A28-R193).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00484-2 is reactive to EPO in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
29 kDa
Calculated molecular weight
52221 MW
Background of Erythropoietin/EPO
This EPO gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00484-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: rat liver tissue, rat kidney tissue, mouse liver tissue, mouse kidney tissue, human HepG2 whole cell, human Caco-2 whole cell
Validation Images & Assay Conditions
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Boster Kit Box
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Figure 1. Western blot analysis of EPO using anti-EPO antibody (A00484-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: mouse liver tissue lysates,
Lane 4: mouse kidney tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPO antigen affinity purified polyclonal antibody (Catalog # A00484-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPO at approximately 29KD. The expected band size for EPO is at 21-34KD.
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Figure 2. Western blot analysis of EPO using anti-EPO antibody (A00484-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPO antigen affinity purified polyclonal antibody (Catalog # A00484-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPO at approximately 29KD. The expected band size for EPO is at 21-34KD.
Protein Target Info & Infographic
Gene/Protein Information For EPO (Source: Uniprot.org, NCBI)
Gene Name
EPO
Full Name
Erythropoietin
Weight
52221 MW
Superfamily
EPO/TPO family
Alternative Names
Erythropoietin; Epoetin; EPO EPO DBAL, ECYT5, EP, MVCD2 erythropoietin erythropoietin|epoetin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EPO, check out the EPO Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EPO: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Erythropoietin EPO Antibody Picoband® (A00484-2)
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Customer Q&As
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17 Customer Q&As for Anti-Erythropoietin EPO Antibody Picoband®
Question
Is this A00484-2 anti-EPO antibody reactive to the isotypes of EPO?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
The immunogen of A00484-2 anti-EPO antibody is E.coli-derived human EPO recombinant protein (Position: A28-R193). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-03
Question
We are currently using anti-EPO antibody A00484-2 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
D. Singh
Verified customer
Asked: 2020-03-26
Answer
The anti-EPO antibody (A00484-2) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-26
Question
My team were content with the WB result of your anti-EPO antibody. However we have observed positive staining in urine secreted. using this antibody. Is that expected? Could you tell me where is EPO supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
From literature, urine does express EPO. Generally EPO expresses in secreted. Regarding which tissues have EPO expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Urine, Pubmed ID: 3949763, 6698989, 3346214, 3219367
Boster Scientific Support
Answered: 2020-03-03
Question
Do you have a BSA free version of anti-EPO antibody A00484-2 available?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-EPO antibody A00484-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-05
Question
We bought anti-EPO antibody for ELISA on right lobe of liver a few months ago. I am using mouse, and We intend to use the antibody for WB next. I would like examining right lobe of liver as well as urine in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
I have checked the website and datasheets of our anti-EPO antibody and I see that A00484-2 has been tested on mouse in both ELISA and WB. Thus A00484-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-02-04
Question
Is a blocking peptide available for product anti-EPO antibody (A00484-2)?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
We do provide the blocking peptide for product anti-EPO antibody (A00484-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-21
Question
I see that the anti-EPO antibody A00484-2 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-29
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for urine using anti-EPO antibody A00484-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-25
Question
I would like using your anti-EPO antibody for positive regulation of activated t cell proliferation studies. Has this antibody been tested with western blotting on mouse liver tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
We appreciate your inquiry. This A00484-2 anti-EPO antibody is tested on rat liver tissue, kidney tissue, mouse liver tissue, human hepg2 whole cell lysates. It is guaranteed to work for ELISA, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-07-05
Question
I have a question about product A00484-2, anti-EPO antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00484-2 anti-EPO antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-29
Question
Would A00484-2 anti-EPO antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-07-26
Answer
It shows on the product datasheet, A00484-2 anti-EPO antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-07-26
Question
Will anti-EPO antibody A00484-2 work on monkey ELISA with urine?
Verified Customer
Verified customer
Asked: 2018-07-17
Answer
Our lab technicians have not tested anti-EPO antibody A00484-2 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-EPO antibody A00484-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey urine in ELISA, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-17
Question
We have been able to see staining in mouse urine. What should we do? Is anti-EPO antibody supposed to stain urine positively?
Verified Customer
Verified customer
Asked: 2018-04-19
Answer
Based on literature urine does express EPO. Based on Uniprot.org, EPO is expressed in right lobe of liver, brain, urine, among other tissues. Regarding which tissues have EPO expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Urine, Pubmed ID: 3949763, 6698989, 3346214, 3219367
Boster Scientific Support
Answered: 2018-04-19
Question
Will anti-EPO antibody A00484-2 work for WB with urine?
Verified Customer
Verified customer
Asked: 2018-04-04
Answer
According to the expression profile of urine, EPO is highly expressed in urine. So, it is likely that anti-EPO antibody A00484-2 will work for WB with urine.
Boster Scientific Support
Answered: 2018-04-04
Question
My lab would like to test anti-EPO antibody A00484-2 on mouse urine for research purposes, then I may be interested in using anti-EPO antibody A00484-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Z. Li
Verified customer
Asked: 2017-08-24
Answer
The products we sell, including anti-EPO antibody A00484-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-24
Question
Here is the WB image, lot number and protocol we used for urine using anti-EPO antibody A00484-2. Please let me know if you require anything else.
K. Anderson
Verified customer
Asked: 2013-07-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-07-25
Question
I was wanting to use your anti-EPO antibody for WB for mouse urine on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse urine identification?
J. Wu
Verified customer
Asked: 2013-02-05
Answer
As indicated on the product datasheet, A00484-2 anti-EPO antibody has been validated for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse urine in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-02-05
