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Product Info Summary
| SKU: | PB9583 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Eph receptor A5/EPHA5 Antibody Picoband®
SKU/Catalog Number
PB9583
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Eph receptor A5/EPHA5 Antibody Picoband® catalog # PB9583. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Eph receptor A5/EPHA5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9583)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Eph receptor A5, different from the related mouse sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9583 is reactive to EPHA5 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
114 kDa
Calculated molecular weight
114803 MW
Background of EPHA5
EPH receptor A5 (ephrin type-A receptor 5) is a protein that in humans is encoded by the EPHA5 gene. This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Alternatively spliced transcript variants encoding different isoforms have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9583 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Positive Control
WB: Rat Brain Tissue, Mouse Brain Tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Eph receptor A5 using anti-Eph receptor A5 antibody (PB9583).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eph receptor A5 antigen affinity purified polyclonal antibody (Catalog # PB9583) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eph receptor A5 at approximately 114 kDa. The expected band size for Eph receptor A5 is at 114 kDa.
Protein Target Info & Infographic
Gene/Protein Information For EPHA5 (Source: Uniprot.org, NCBI)
Gene Name
EPHA5
Full Name
Ephrin type-A receptor 5
Weight
114803 MW
Superfamily
protein kinase superfamily
Alternative Names
Ephrin type-A receptor 5;2.7.10.1;Brain-specific kinase;EPH homology kinase 1;EHK-1;EPH-like kinase 7;EK7;hEK7;EPHA5;BSK, EHK1, HEK7, TYRO4; EPHA5 CEK7, EHK-1, EHK1, EK7, HEK7, TYRO4 EPH receptor A5 ephrin type-A receptor 5|EPH homology kinase 1|EPH-like kinase 7|brain-specific kinase|epididymis secretory sperm binding protein|receptor protein-tyrosine kinase HEK7|tyrosine-protein kinase receptor EHK-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EPHA5, check out the EPHA5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EPHA5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Eph receptor A5/EPHA5 Antibody Picoband® (PB9583)
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1 Customer Q&As for Anti-Eph receptor A5/EPHA5 Antibody Picoband®
Question
We are currently using anti-Eph receptor A5/EPHA5 antibody PB9583 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-06-06
Answer
The anti-Eph receptor A5/EPHA5 antibody (PB9583) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-06
