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Product Info Summary
| SKU: | M30950-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-EpCAM/Trop1 Rabbit Monoclonal Antibody
View all EpCAM/TROP1 Antibodies
SKU/Catalog Number
M30950-2
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-EpCAM/Trop1 Rabbit Monoclonal Antibody catalog # M30950-2. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EpCAM/Trop1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30950-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GBG-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human EpCAM
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M30950-2 is reactive to EPCAM in Human
Reconstitution
Observed Molecular Weight
50 kDa
Calculated molecular weight
34932 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M30950-2 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
Positive Control
IHC: human stomach carcinoma tissue
Validation Images & Assay Conditions
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Western blot analysis of EpCAM expression in A431 cell lysate.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human stomach carcinoma, using EpCAM Antibody.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For EPCAM (Source: Uniprot.org, NCBI)
Gene Name
EPCAM
Full Name
Epithelial cell adhesion molecule
Weight
34932 MW
Superfamily
EPCAM family
Alternative Names
Epithelial cell adhesion molecule;Ep-CAM;Adenocarcinoma-associated antigen;Cell surface glycoprotein Trop-1;Epithelial cell surface antigen;Epithelial glycoprotein;EGP;Epithelial glycoprotein 314;EGP314;hEGP314;KS 1/4 antigen;KSA;Major gastrointestinal tumor-associated protein GA733-2;Tumor-associated calcium signal transducer 1;CD326;EPCAM;GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1; EPCAM DIAR5, EGP-2, EGP314, EGP40, ESA, HNPCC8, KS1/4, KSA, M4S1, MIC18, MK-1, TACSTD1, TROP1 epithelial cell adhesion molecule epithelial cell adhesion molecule|adenocarcinoma-associated |cell surface glycoprotein Trop-1|epithelial glycoprotein 314|human epithelial glycoprotein-2|major gastrointestinal tumor-associated protein GA733-2|membrane component, chromosome 4, surface marker (35kD glycoprotein)|trophoblast cell surface 1|tumor-associated calcium signal transducer 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EPCAM, check out the EPCAM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EPCAM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EpCAM/Trop1 Rabbit Monoclonal Antibody (M30950-2)
Hello CJ!
M30950-2 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
A novel multi-target RNAi adenovirus inhibits hepatoma cell proliferation, migration, and induction of angiogenesis
Zhang Q, Bu S, Sun J, Xu M, Yao X, He K, Lai D. Stem Cell Res Ther. 2017 Nov 28;8(1):270. doi: 10.1186/s13287-017-0721-0. Paracrine effects of human amniotic epithelial cells protect against chemotherapy-induced ovarian damage
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-EpCAM/Trop1 Rabbit Monoclonal Antibody
Question
We have seen staining in human colon carcinoma. Any tips? Is anti-EpCAM/Trop1 Rabbit Monoclonal antibody supposed to stain colon carcinoma positively?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
From literature colon carcinoma does express EPCAM. From Uniprot.org, EPCAM is expressed in jejunal mucosa, lung adenocarcinoma, colon carcinoma, lymphoma, ovary, placenta, liver, among other tissues. Regarding which tissues have EPCAM expression, here are a few articles citing expression in various tissues:
Colon carcinoma, Pubmed ID: 2333300
Liver, Pubmed ID: 19159218
Lung adenocarcinoma, Pubmed ID: 2463074, 2469722, 2108441
Lymphoma, Pubmed ID: 8382772
Ovary, Pubmed ID: 15489334
Placenta, Pubmed ID: 2911574
Boster Scientific Support
Answered: 2020-04-30
Question
I see that the anti-EpCAM/Trop1 Rabbit Monoclonal antibody M30950-2 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-01
Question
We are currently using anti-EpCAM/Trop1 Rabbit Monoclonal antibody M30950-2 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
The anti-EpCAM/Trop1 Rabbit Monoclonal antibody (M30950-2) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-03
Question
I am interested in using your anti-EpCAM/Trop1 Rabbit Monoclonal antibody for with tufting enteropathy studies. Has this antibody been tested with western blotting on a431 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
I appreciate your inquiry. This M30950-2 anti-EpCAM/Trop1 Rabbit Monoclonal antibody is validated on a431 cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-08
Question
My team were content with the WB result of your anti-EpCAM/Trop1 Rabbit Monoclonal antibody. However we have observed positive staining in jejunal mucosa lateral cell membrane using this antibody. Is that expected? Could you tell me where is EPCAM supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
Based on literature, jejunal mucosa does express EPCAM. Generally EPCAM expresses in lateral cell membrane. Regarding which tissues have EPCAM expression, here are a few articles citing expression in various tissues:
Colon carcinoma, Pubmed ID: 2333300
Liver, Pubmed ID: 19159218
Lung adenocarcinoma, Pubmed ID: 2463074, 2469722, 2108441
Lymphoma, Pubmed ID: 8382772
Ovary, Pubmed ID: 15489334
Placenta, Pubmed ID: 2911574
Boster Scientific Support
Answered: 2019-10-14
Question
Can you help my question with product M30950-2, anti-EpCAM/Trop1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M30950-2 anti-EpCAM/Trop1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-25
Question
Is a blocking peptide available for product anti-EpCAM/Trop1 Rabbit Monoclonal antibody (M30950-2)?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
We do provide the blocking peptide for product anti-EpCAM/Trop1 Rabbit Monoclonal antibody (M30950-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-18
Question
I am interested in to test anti-EpCAM/Trop1 Rabbit Monoclonal antibody M30950-2 on human ovary for research purposes, then I may be interested in using anti-EpCAM/Trop1 Rabbit Monoclonal antibody M30950-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
The products we sell, including anti-EpCAM/Trop1 Rabbit Monoclonal antibody M30950-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-29
Question
I was wanting to use your anti-EpCAM/Trop1 Rabbit Monoclonal antibody for WB for human ovary on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human ovary identification?
P. Miller
Verified customer
Asked: 2019-04-22
Answer
You can see on the product datasheet, M30950-2 anti-EpCAM/Trop1 Rabbit Monoclonal antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human ovary in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-22
Question
Is this M30950-2 anti-EpCAM/Trop1 Rabbit Monoclonal antibody reactive to the isotypes of EPCAM?
Verified Customer
Verified customer
Asked: 2018-09-27
Answer
The immunogen of M30950-2 anti-EpCAM/Trop1 Rabbit Monoclonal antibody is A synthesized peptide derived from human EpCAM . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-09-27
Question
I have attached the WB image, lot number and protocol we used for ovary using anti-EpCAM/Trop1 Rabbit Monoclonal antibody M30950-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-09-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-21
Question
Do you have a BSA free version of anti-EpCAM/Trop1 Rabbit Monoclonal antibody M30950-2 available?
Verified Customer
Verified customer
Asked: 2018-07-19
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-EpCAM/Trop1 Rabbit Monoclonal antibody M30950-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-07-19
Question
Would anti-EpCAM/Trop1 Rabbit Monoclonal antibody M30950-2 work for WB with ovary?
J. Mangal
Verified customer
Asked: 2016-11-14
Answer
According to the expression profile of ovary, EPCAM is highly expressed in ovary. So, it is likely that anti-EpCAM/Trop1 Rabbit Monoclonal antibody M30950-2 will work for WB with ovary.
Boster Scientific Support
Answered: 2016-11-14
Question
Will anti-EpCAM/Trop1 Rabbit Monoclonal antibody M30950-2 work on canine WB with liver?
C. Moore
Verified customer
Asked: 2016-04-21
Answer
Our lab technicians have not tested anti-EpCAM/Trop1 Rabbit Monoclonal antibody M30950-2 on canine. You can run a BLAST between canine and the immunogen sequence of anti-EpCAM/Trop1 Rabbit Monoclonal antibody M30950-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-04-21
Question
Does M30950-2 anti-EpCAM/Trop1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
H. Parker
Verified customer
Asked: 2013-09-03
Answer
As indicated on the product datasheet, M30950-2 anti-EpCAM/Trop1 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-09-03
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for ovary using anti-EpCAM/Trop1 Rabbit Monoclonal antibody M30950-2. Let me know if you need anything else.
A. Jackson
Verified customer
Asked: 2013-04-15
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-04-15
