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Product Info Summary
| SKU: | A02483-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Epac1/RAPGEF3 Antibody Picoband®
SKU/Catalog Number
A02483-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Epac1/RAPGEF3 Antibody Picoband® catalog # A02483-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Epac1/RAPGEF3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02483-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Epac1/RAPGEF3 recombinant protein (Position: E451-D698).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02483-2 is reactive to RAPGEF3 in Human, Monkey, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
104 kDa
Calculated molecular weight
22105 MW
Background of RAPGEF3
Rap guanine nucleotide exchange factor 3 also known as exchange factor directly activated by cAMP 1 (EPAC1) or cAMP-regulated guanine nucleotide exchange factor I (cAMP-GEFI) is a protein that in humans is encoded by the RAPGEF3 gene. As the name suggests, EPAC proteins (EPAC1 and EPAC2) are a family of intracellular sensors for cAMP, and function as nucleotide exchange factors for the Rap subfamily of RAS-like small GTPases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02483-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat, Monkey
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human HELA whole cell, human placenta tissue, monkey kidney tissue, rat kidney tissue, rat lung tissue, mouse kidney tissue
FCM: U87 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Epac1/RAPGEF3 using anti-Epac1/RAPGEF3 antibody (A02483-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HELA whole cell lysates,
Lane 2: human placenta tissue lysates,
Lane 3: monkey kidney tissue lysates,
Lane 4: rat kidney tissue lysates,
Lane 5: rat lung tissue lysates,
Lane 6: mouse kidney tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Epac1/RAPGEF3 antigen affinity purified polyclonal antibody (Catalog # A02483-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Epac1/RAPGEF3 at approximately 104KD. The expected band size for Epac1/RAPGEF3 is at 104KD.
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Figure 2. Flow Cytometry analysis of U87 cells using anti-Epac1/RAPGEF3 antibody (A02483-2).
Overlay histogram showing U87 cells stained with A02483-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Epac1/RAPGEF3 Antibody (A02483-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For RAPGEF3 (Source: Uniprot.org, NCBI)
Gene Name
RAPGEF3
Full Name
Rap guanine nucleotide exchange factor 3
Weight
22105 MW
Alternative Names
Fatty acid-binding protein, liver; Fatty acid-binding protein 1; Liver-type fatty acid-binding protein; L-FABP; Squalene- and sterol-carrier protein; SCP; Z-protein; p14; Fabp1 RAPGEF3 CAMP-GEFI, EPAC, EPAC1, HSU79275, bcm910 Rap guanine nucleotide exchange factor 3 rap guanine nucleotide exchange factor 3|9330170P05Rik|EPAC 1|Rap guanine nucleotide exchange factor (GEF) 3|Rap1 guanine-nucleotide-exchange factor directly activated by cAMP|cAMP-regulated guanine nucleotide exchange factor I|exchange factor directly activated by cAMP 1|exchange protein directly activated by cAMP 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAPGEF3, check out the RAPGEF3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAPGEF3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Epac1/RAPGEF3 Antibody Picoband® (A02483-2)
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