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Product Info Summary
| SKU: | A02483 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-EPAC1 RAPGEF3 Antibody
SKU/Catalog Number
A02483
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-EPAC1 RAPGEF3 Antibody (Catalog # A02483). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
EPAC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-EPAC1 RAPGEF3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02483)
Host
Rabbit
Contents
EPAC1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
EPAC1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human EPAC1. The immunogen is located within amino acids 60 - 110 of EPAC1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms of EPAC1 are known to exist; this antibody will detect both isoforms. EPAC1 antibody is predicted to not cross-react with EPAC2.
Reactive Species
A02483 is reactive to RAPGEF3 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
103751 MW
Background of RAPGEF3
EPAC1, also known as Rap guanine nuclear exchange factor 3 and cAMPGEF-I, is widely expressed but most prominently in brain, heart, kidney, pancreas, spleen, ovary, thyroid and spinal cord. EPAC1 is a cAMP-binding protein with intrinsic guanine nuclear exchange factor activity that couples cAMP production to the activation of Rap, a GTPase belonging to the Ras family. This activation of Rap influences numerous cellular processes such as integrin-mediated cell adhesion, vascular endothelial barrier formation, and cardiac myocyte gap junction formation. Recently, EPAC1 has been suggested to also be involved in the cAMP-dependent regulation of ion channel formation, intracellular Ca++ signalling, ion transporter activity, and exocytosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02483 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
EPAC1 antibody can be used for detection of EPAC1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of EPAC1 in rat skeletal muscle tissue lysate with EPAC1 antibody at 1 μg/mL.
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Immunohistochemistry of EPAC1 in rat brain tissue with EPAC1 antibody at 2.5 μg/mL.
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Immunofluorescence of EPAC1 in rat brain tissue with EPAC1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For RAPGEF3 (Source: Uniprot.org, NCBI)
Gene Name
RAPGEF3
Full Name
Rap guanine nucleotide exchange factor 3
Weight
103751 MW
Alternative Names
EPAC, EPAC1, bcm910, HSU79275, CAMP-GEFI, CGEF1, EPAC, Rap guanine nucleotide exchange factor 3, Exchange factor directly activated by cAMP 1, EPAC 1 RAPGEF3 CAMP-GEFI, EPAC, EPAC1, HSU79275, bcm910 Rap guanine nucleotide exchange factor 3 rap guanine nucleotide exchange factor 3|9330170P05Rik|EPAC 1|Rap guanine nucleotide exchange factor (GEF) 3|Rap1 guanine-nucleotide-exchange factor directly activated by cAMP|cAMP-regulated guanine nucleotide exchange factor I|exchange factor directly activated by cAMP 1|exchange protein directly activated by cAMP 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAPGEF3, check out the RAPGEF3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAPGEF3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EPAC1 RAPGEF3 Antibody (A02483)
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