Click image to see more details
Product Info Summary
| SKU: | M02483 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Epac1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02483
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Epac1 Rabbit Monoclonal Antibody catalog # M02483. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Epac1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02483)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AEOG-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Epac1 The activation of RaP1 by cAMP is independent of PKA and is mediated by recently discovered family of guanine nucleotide exchange factors (GEFs) called cAMP-GEFs or Epacs. The Epac signaling therefore represents a novel mechanism for cAMP signaling with in the cAMP cascade.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02483 is reactive to RAPGEF3 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
104 kDa
Calculated molecular weight
104 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02483 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: human HUVEC whole cell, rat brain tissue, rat kidney tissue, mouse brain tissue, mouse kidney tissue<>brIHC: mouse brain tissue, rat brain tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Epac1 using anti-Epac1 antibody (M02483).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HUVEC whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Epac1 antigen affinity purified monoclonal antibody (Catalog # M02483) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Epac1 at approximately 104 kDa. The expected band size for Epac1 is at 104 kDa.
Click image to see more details
Figure 2. IHC analysis of Epac1 using anti-Epac1 antibody (M02483).
Epac1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-Epac1 Antibody (M02483) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Epac1 using anti-Epac1 antibody (M02483).
Epac1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-Epac1 Antibody (M02483) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For RAPGEF3 (Source: Uniprot.org, NCBI)
Gene Name
RAPGEF3
Full Name
Rap guanine nucleotide exchange factor 3
Weight
104 kDa
Alternative Names
Fatty acid-binding protein, liver;Fatty acid-binding protein 1;Liver-type fatty acid-binding protein;L-FABP;FABP1;FABPL; RAPGEF3 CAMP-GEFI, EPAC, EPAC1, HSU79275, bcm910 Rap guanine nucleotide exchange factor 3 rap guanine nucleotide exchange factor 3|9330170P05Rik|EPAC 1|Rap guanine nucleotide exchange factor (GEF) 3|Rap1 guanine-nucleotide-exchange factor directly activated by cAMP|cAMP-regulated guanine nucleotide exchange factor I|exchange factor directly activated by cAMP 1|exchange protein directly activated by cAMP 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAPGEF3, check out the RAPGEF3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAPGEF3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Epac1 Rabbit Monoclonal Antibody (M02483)
Hello CJ!
No publications found for M02483
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Epac1 Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Epac1 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-Epac1 Rabbit Monoclonal Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-Epac1 Monoclonal antibody M02483. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-07
Question
I see that the anti-Epac1 Monoclonal antibody M02483 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-04
Question
I was wanting to use your anti-Epac1 Monoclonal antibody for IP for mouse kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse kidney identification?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
As indicated on the product datasheet, M02483 anti-Epac1 Monoclonal antibody has been validated for IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-29
Question
Will M02483 anti-Epac1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-08-31
Answer
It shows on the product datasheet, M02483 anti-Epac1 Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-08-31
Question
We are currently using anti-Epac1 Monoclonal antibody M02483 for rat tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
J. Yang
Verified customer
Asked: 2015-11-12
Answer
The anti-Epac1 Monoclonal antibody (M02483) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-11-12
