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Product Info Summary
| SKU: | A06845-2 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-ENOB Antibody (Center)
SKU/Catalog Number
A06845-2
Size
80 µl
Description
Boster Bio Anti-ENOB Antibody (Center) (Catalog # A06845-2). Tested in WB, IHC-P, Flow Cytometry application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ENOB Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06845-2)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This ENOB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-264 amino acids from the Central region of human ENOB.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06845-2 is reactive to ENO3 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
46987 Da
Background of ENO3
ENOB is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in its gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06845-2 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:50-1:100
FC: 1:10-1:50
Validation Images & Assay Conditions
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ENOB Antibody (Center) western blot analysis in RD cell line and mouse skeletal muscle,rat skeletal muscle lysates (35ug/lane).This demonstrates the ENOB antibody detected the ENOB protein (arrow).
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Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ENOB Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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ENOB Antibody (Center) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For ENO3 (Source: Uniprot.org, NCBI)
Gene Name
ENO3
Full Name
Beta-enolase
Weight
46987 Da
Superfamily
enolase family
Alternative Names
Beta-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 3, Muscle-specific enolase, MSE, Skeletal muscle enolase, ENO3 ENO3 GSD13, MSE enolase 3 beta-enolase|2-phospho-D-glycerate hydrolyase|enolase 3 (beta, muscle)|muscle enriched enolase|muscle-specific enolase|skeletal muscle enolase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ENO3, check out the ENO3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ENO3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ENOB Antibody (Center) (A06845-2)
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