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Product Info Summary
| SKU: | A01828-4 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Endothelin A Receptor/ET-A/EDNRA Antibody Picoband®
View all EDNRA/Endothelin R Type A Antibodies
SKU/Catalog Number
A01828-4
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Endothelin A Receptor/ET-A/EDNRA Antibody Picoband® catalog # A01828-4. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Endothelin A Receptor/ET-A/EDNRA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01828-4)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Endothelin A Receptor/ET-A/EDNRA recombinant protein (Position: N32-Q381).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01828-4 is reactive to EDNRA in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
50 kDa
Calculated molecular weight
18080 MW
Background of EDNRA/Endothelin R Type A
This gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01828-4 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human HEK293 whole cell
IHC: human lung cancer tissue
FCM: A549 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Endothelin A Receptor/ET-A/ EDNRA using anti-Endothelin A Receptor/ET-A/ EDNRA antibody (A01828-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Endothelin A Receptor/ET-A/ EDNRA antigen affinity purified polyclonal antibody (Catalog # A01828-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Endothelin A Receptor/ET-A/ EDNRA at approximately 50KD. The expected band size for Endothelin A Receptor/ET-A/ EDNRA is at 50KD.
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Figure 2. IHC analysis of Endothelin A Receptor/ET-A/ EDNRA using anti-Endothelin A Receptor/ET-A/ EDNRA antibody (A01828-4).
Endothelin A Receptor/ET-A/ EDNRA was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Endothelin A Receptor/ET-A/ EDNRA Antibody (A01828-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. Flow Cytometry analysis of A549 cells using anti-Endothelin A Receptor/ET-A/ EDNRA antibody (A01828-4).
Overlay histogram showing A549 cells stained with A01828-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Endothelin A Receptor/ET-A/ EDNRA Antibody (A01828-4,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For EDNRA (Source: Uniprot.org, NCBI)
Gene Name
EDNRA
Full Name
Endothelin-1 receptor
Weight
18080 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Thy-1 membrane glycoprotein; CDw90; Thy-1 antigen; CD90; THY1 EDNRA ET-A, ETA, ETA-R, ETAR, ETRA, MFDA, hET-AR endothelin receptor type A endothelin-1 receptor|G protein-coupled receptor|endothelin receptor subtype A|endothelin-1-specific receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EDNRA, check out the EDNRA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EDNRA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Endothelin A Receptor/ET-A/EDNRA Antibody Picoband® (A01828-4)
Hello CJ!
A01828-4 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Peng Hj, Dai Dz, Ji H, Dai Y. Eur J Pharmacol. 2010 May 25;634(1-3):101-6. Doi: 10.1016/J.Ejphar.2010.02.001. Epub 2010 Feb 16. The Separate Roles Of Endothelin Receptors Participate In Remodeling Of Matrix Metalloproteinase And Connexin 43 Of Car...
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