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Product Info Summary
| SKU: | A01828-3 |
|---|---|
| Size: | 250µg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Sheep |
| Application: | IHC |
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Product info
Product Name
Anti-Endothelin A Receptor Ednra Antibody
View all EDNRA/Endothelin R Type A Antibodies
SKU/Catalog Number
A01828-3
Size
250µg
Form
Liquid. In TRIS-citrate-phosphate, pH 7-8, containing 0.1% sodium azide.
Description
Sheep Polyclonal antibody for Ednra {ECO:0000312 detection. Tested positive for IHC, WB in Human, Mouse, Rat
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Endothelin A Receptor Ednra Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01828-3)
Host
Sheep
Contents
Liquid. In TRIS-citrate-phosphate, pH 7-8, containing 0.1% sodium azide.
Clonality
Polyclonal
Immunogen
Synthetic peptide corresponding to aa 410-422 (Q410EQNHNTERSSHK422) of C-terminal rat ETA-receptor (endothelin A receptor) .
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01828-3 is reactive to Ednra in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
48.722kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01828-3 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (paraffin sections, 4µg/ml)
Western Blot (10-20µg/ml using ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Validation Images & Assay Conditions
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Figure 1. IHC analysis of Ednra using anti-Ednra antibody (A01828-3).
Ednra was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Ednra Antibody (A01828-3) overnight at 4°C. Biotinylated goat anti Sheep IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1023) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Ednra (Source: Uniprot.org, NCBI)
Gene Name
Ednra
Full Name
Endothelin-1 receptor
Weight
48.722kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
EDNRA; endothelin receptor type A; endothelin-1 receptor; endothelin-1-specific receptor; ET1-specific type; ETAR; ETRA; G protein-coupled receptor; hET-AR EDNRA ET-A, ETA, ETA-R, ETAR, ETRA, MFDA, hET-AR endothelin receptor type A endothelin-1 receptor|G protein-coupled receptor|endothelin receptor subtype A|endothelin-1-specific receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ednra, check out the Ednra Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ednra: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Endothelin A Receptor Ednra Antibody (A01828-3)
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Customer Q&As
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5 Customer Q&As for Anti-Endothelin A Receptor Ednra Antibody
Question
Do you have a BSA free version of anti-Endothelin A Receptor antibody A01828-3 available?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Endothelin A Receptor antibody A01828-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-21
Question
Does anti-Endothelin A Receptor antibody A01828-3 work on pig IHC with cerebellum?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
Our lab technicians have not tested anti-Endothelin A Receptor antibody A01828-3 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Endothelin A Receptor antibody A01828-3 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig cerebellum in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-22
Question
I have a question about product A01828-3, anti-Endothelin A Receptor antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-02-15
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01828-3 anti-Endothelin A Receptor antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-02-15
Question
We are currently using anti-Endothelin A Receptor antibody A01828-3 for mouse tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
R. Miller
Verified customer
Asked: 2016-03-09
Answer
The anti-Endothelin A Receptor antibody (A01828-3) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-03-09
Question
Is this A01828-3 anti-Endothelin A Receptor antibody reactive to the isotypes of EDNRA?
P. Gonzalez
Verified customer
Asked: 2014-08-18
Answer
The immunogen of A01828-3 anti-Endothelin A Receptor antibody is Synthetic peptide corresponding to aa 410-422 (Q410EQNHNTERSSHK422) of C-terminal rat ETA-receptor (endothelin A receptor) . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-08-18
