Anti-Endothelin A Receptor Ednra Antibody

Product Name

Anti-Endothelin A Receptor Ednra Antibody

View all EDNRA/Endothelin R Type A Antibodies

SKU/Catalog Number

A01828-3

Size

250µg

Form

Liquid. In TRIS-citrate-phosphate, pH 7-8, containing 0.1% sodium azide.

Description

Sheep Polyclonal antibody for Ednra {ECO:0000312 detection. Tested positive for IHC, WB in Human, Mouse, Rat

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Endothelin A Receptor Ednra Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01828-3)

Host

Sheep

Contents

Liquid. In TRIS-citrate-phosphate, pH 7-8, containing 0.1% sodium azide.

Clonality

Polyclonal

Immunogen

Synthetic peptide corresponding to aa 410-422 (Q⁴¹⁰EQNHNTERSSHK⁴²²)  of C-terminal rat ET_A-receptor (endothelin A receptor) .

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A01828-3 is reactive to EDNRA in Human, Mouse, Rat

Applications

A01828-3 is guaranteed for IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (paraffin sections, 4µg/ml)
Western Blot (10-20µg/ml using ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.

Validation Images & Assay Conditions

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Figure 1. IHC analysis of Ednra using anti-Ednra antibody (A01828-3).
Ednra was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Ednra Antibody (A01828-3) overnight at 4°C. Biotinylated goat anti Sheep IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1023) with DAB as the chromogen.

Gene/Protein Information For EDNRA (Source: Uniprot.org, NCBI)

Alternative Names

EDNRA; endothelin receptor type A; endothelin-1 receptor; endothelin-1-specific receptor; ET1-specific type; ETAR; ETRA; G protein-coupled receptor; hET-AR EDNRA ET-A, ETA, ETA-R, ETAR, ETRA, MFDA, hET-AR endothelin receptor type A endothelin-1 receptor|G protein-coupled receptor|endothelin receptor subtype A|endothelin-1-specific receptor

For more info on EDNRA, check out the EDNRA Infographic

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In this infographic, you will see the following information for EDNRA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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