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Product Info Summary
| SKU: | M00135-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-eIF4E Monoclonal Antibody
SKU/Catalog Number
M00135-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-eIF4E Monoclonal Antibody catalog # M00135-1. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-eIF4E Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00135-1)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
5D11
Isotype
IgG
Immunogen
Purified recombinant fragment of human eIF4E expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00135-1 is reactive to EIF4E in Human
Reconstitution
Calculated molecular weight
25097 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00135-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
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Figure 5. ELISA validation of EIF4E using Anti-eIF4E Monoclonal Antibody (M00135-1).
ELISA analysis of eIF4E antibody.
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Figure 4. Flow Cytometry validation of EIF4E using Anti-eIF4E Monoclonal Antibody (M00135-1).
Flow cytometric (FCM) analysis of HeLa cells using eIF4E Monoclonal Antibody (green) and negative control (purple).
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Figure 3. Immunofluorescent staining data of EIF4E using Anti-eIF4E Monoclonal Antibody (M00135-1).
Immunofluorescence (IF) analysis of GC-7901 cells using eIF4E Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Figure 2. Immunohistochemistry validation of EIF4E using Anti-eIF4E Monoclonal Antibody (M00135-1).
Immunohistochemistry (IHC) analysis of paraffin-embedded liver cancer (left) and submaxillary tumor (right) with DAB staining using eIF4E Monoclonal Antibody.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-eIF4E Monoclonal Antibody M00135-1
Western Blot (WB) analysis using eIF4E Monoclonal Antibody against HeLa (1)Protein Target Info & Infographic
Gene/Protein Information For EIF4E (Source: Uniprot.org, NCBI)
Gene Name
EIF4E
Full Name
Eukaryotic translation initiation factor 4E
Weight
25097 MW
Superfamily
eukaryotic initiation factor 4E family
Alternative Names
Eukaryotic translation initiation factor 4E;eIF-4E;eIF4E;eIF-4F 25 kDa subunit;mRNA cap-binding protein;EIF4E;EIF4EL1, EIF4F; EIF4E AUTS19, CBP1, EIF4EL1, EIF4F, eIF-4E, EIF4E eukaryotic translation initiation factor 4E eukaryotic translation initiation factor 4E|eIF-4F 25 kDa subunit|eukaryotic translation initiation factor 4E-like 1|mRNA cap-binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EIF4E, check out the EIF4E Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF4E: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-eIF4E Monoclonal Antibody (M00135-1)
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