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Product Info Summary
| SKU: | M00135 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IHC, WB |
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Product info
Product Name
Anti-EIF4E Monoclonal Antibody
SKU/Catalog Number
M00135
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-EIF4E Monoclonal Antibody catalog # M00135. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EIF4E Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00135)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFHI-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human EIF4E eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00135 is reactive to EIF4E in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
25 kDa
Calculated molecular weight
82785 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00135 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:100-1:5000
IHC 1:100-1:500
IP 1:30
FC 1:40
Positive Control
WB: human 293T whole cell, human Hela whole cell, human HepG2 whole cell, human A549 whole cell, rat RH35 whole cell, rat PC-12 whole cell, mouse HEPA1-2 whole cell, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of EIF4E using anti-EIF4E antibody (M00135).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat RH35 whole cell lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse HEPA1-2 whole cell lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF4E antigen affinity purified monoclonal antibody (Catalog # M00135) at 1:100 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF4E at approximately 25 kDa. The expected band size for EIF4E is at 25 kDa.
Protein Target Info & Infographic
Gene/Protein Information For EIF4E (Source: Uniprot.org, NCBI)
Gene Name
EIF4E
Full Name
Eukaryotic translation initiation factor 4E
Weight
82785 MW
Superfamily
eukaryotic initiation factor 4E family
Alternative Names
Transcriptional repressor CTCF;11-zinc finger protein;CCCTC-binding factor;CTCFL paralog;CTCF; EIF4E AUTS19, CBP1, EIF4EL1, EIF4F, eIF-4E, EIF4E eukaryotic translation initiation factor 4E eukaryotic translation initiation factor 4E|eIF-4F 25 kDa subunit|eukaryotic translation initiation factor 4E-like 1|mRNA cap-binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EIF4E, check out the EIF4E Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF4E: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EIF4E Monoclonal Antibody (M00135)
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No publications found for M00135
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Customer Q&As
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4 Customer Q&As for Anti-EIF4E Monoclonal Antibody
Question
Would M00135 react with Arabidopsis samples?
Verified customer
Asked: 2019-09-14
Answer
We haven't tested the Anti-EIF4E Monoclonal Antibody (M00135) on Arabidopsis samples. Please run a pilot test. Immunogen sequence for this antibody is CVTHIGRVYKERLGLPPKIV.
Boster Scientific Support
Answered: 2019-09-17
Question
Do you have a BSA free version of anti-EIF4E Monoclonal antibody M00135 available?
E. Edwards
Verified customer
Asked: 2016-02-23
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-EIF4E Monoclonal antibody M00135 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-02-23
Question
Is this M00135 anti-EIF4E Monoclonal antibody reactive to the isotypes of EIF4E?
R. Kulkarni
Verified customer
Asked: 2016-01-12
Answer
The immunogen of M00135 anti-EIF4E Monoclonal antibody is A synthesized peptide derived from human EIF4E. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-01-12
Question
We are interested in to test anti-EIF4E Monoclonal antibody M00135 on rat erythroleukemia for research purposes, then I may be interested in using anti-EIF4E Monoclonal antibody M00135 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
V. Brown
Verified customer
Asked: 2015-09-16
Answer
The products we sell, including anti-EIF4E Monoclonal antibody M00135, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-09-16
