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Product Info Summary
| SKU: | A06212-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-eIF3 Zeta (R135) EIF3D Antibody
SKU/Catalog Number
A06212-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-eIF3 Zeta (R135) EIF3D Antibody catalog # A06212-1. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-eIF3 Zeta (R135) EIF3D Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06212-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids 111-160 of Human eIF3ζ.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06212-1 is reactive to EIF3D in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Calculated molecular weight
63973 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06212-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of EIF3D using Anti-eIF3 Zeta (R135) EIF3D Antibody (A06212-1).
Immunohistochemistry (IHC) analyzes of eIF3ζ (R135) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-eIF3 Zeta (R135) EIF3D Antibody A06212-1
Western blot (WB) analysis of eIF3ζ (R135) pAb at 1:1000 dilutionLane1:The Brain tissue lysate of Mouse(40ug)
Lane2:The Brain tissue lysate of Rat(20ug)
Lane3:HEK293T whole cell lysate(10ug)
Lane4:MCF-7 whole cell lysate(20ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For EIF3D (Source: Uniprot.org, NCBI)
Gene Name
EIF3D
Full Name
Eukaryotic translation initiation factor 3 subunit D
Weight
63973 MW
Superfamily
eIF-3 subunit D family
Alternative Names
Eukaryotic translation initiation factor 3 subunit D EIF3D EIF3S7, eIF3-p66, eIF3-zeta eukaryotic translation initiation factor 3 subunit D eukaryotic translation initiation factor 3 subunit D|eIF3 p66|eukaryotic translation initiation factor 3, subunit 7 zeta, 66/67kDa|translation initiation factor eIF3 p66 subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EIF3D, check out the EIF3D Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF3D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-eIF3 Zeta (R135) EIF3D Antibody (A06212-1)
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