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Product Info Summary
| SKU: | A02345-1 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Sheep |
| Application: | IHC |
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Product info
Product Name
Anti-Stromal interaction molecule 2 Stim2 Antibody
SKU/Catalog Number
A02345-1
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Stromal interaction molecule 2 Stim2 Antibody (Catalog # A02345-1). Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Stromal interaction molecule 2 Stim2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02345-1)
Host
Sheep
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 30% Glycerol
Clonality
Polyclonal
Isotype
IgG
Immunogen
Stim II affinity purified antibody was prepared from whole sheep serum produced by repeated immunizations with a synthetic peptide near the C-terminus of human Stim II.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02345-1 is reactive to STIM2 in Human
Applications
A02345-1 is guaranteed for IHC Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
Background of STIM2
Stim II antibody functions as a calcium sensor in the endoplasmic reticulum via its EF-hand domain. After depletion of Ca+2, it is thought to translocate from the endoplasmic reticulum to the plasma membrane to activate the calcium release-activated channel subunit. It may inhibit STIM1-mediated calcium influx. Anti-Stim II antibody is ideal for investigators interested in Signal Transduction research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1: 10,000
IHC: 5 µg/mL
WB: 1ug/ml
Validation Images & Assay Conditions
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Figure 1. IHC analysis of STIM2 using anti-STIM2 antibody (A02345-1).
STIM2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-STIM2 Antibody (A02345-1) overnight at 4°C. Biotinylated goat anti Sheep IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1023) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For STIM2 (Source: Uniprot.org, NCBI)
Gene Name
STIM2
Full Name
Stromal interaction molecule 2
Weight
Alternative Names
FLJ39527; KIAA1482; stromal interaction molecule 2 STIM2 stromal interaction molecule 2 stromal interaction molecule 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STIM2, check out the STIM2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STIM2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Stromal interaction molecule 2 Stim2 Antibody (A02345-1)
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Customer Q&As
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7 Customer Q&As for Anti-Stromal interaction molecule 2 Stim2 Antibody
Question
Here is the WB image, lot number and protocol we used for fetal brain kidney using anti-Stim2 antibody A02345-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-27
Question
We are currently using anti-Stim2 antibody A02345-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
The anti-Stim2 antibody (A02345-1) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-23
Question
Is a blocking peptide available for product anti-Stim2 antibody (A02345-1)?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
We do provide the blocking peptide for product anti-Stim2 antibody (A02345-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-20
Question
Will A02345-1 anti-Stim2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-02-19
Answer
It shows on the product datasheet, A02345-1 anti-Stim2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-02-19
Question
I was wanting to use your anti-Stim2 antibody for WB for human fetal brain kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human fetal brain kidney identification?
Verified Customer
Verified customer
Asked: 2017-12-01
Answer
As indicated on the product datasheet, A02345-1 anti-Stim2 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human fetal brain kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-01
Question
I see that the anti-Stim2 antibody A02345-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-10-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-10-12
Question
Will anti-Stim2 antibody A02345-1 work on horse WB with brain?
L. Li
Verified customer
Asked: 2014-12-18
Answer
Our lab technicians have not validated anti-Stim2 antibody A02345-1 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Stim2 antibody A02345-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-12-18
