Click image to see more details
Product Info Summary
| SKU: | A00921-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-EGR2 Antibody Picoband®
SKU/Catalog Number
A00921-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-EGR2 Antibody Picoband® catalog # A00921-1. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-EGR2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00921-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human EGR2 recombinant protein (Position: M1-R257). Human EGR2 shares 90.7% amino acid (aa) sequence identity with both mouse and rat EGR2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00921-1 is reactive to EGR2 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
50 kDa
Calculated molecular weight
50,45 kDa
Background of EGR2
Early growth response protein 2 is a protein that in humans is encoded by the EGR2 gene. The protein encoded by this gene is a transcription factor with three tandem C2H2-type zinc fingers. Defects in this gene are associated with Charcot-Marie-Tooth disease type 1D (CMT1D), Charcot-Marie-Tooth disease type 4E (CMT4E), and with Dejerine-Sottas syndrome (DSS). Multiple transcript variants encoding two different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00921-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human PC-3 whole cell, human U251 whole cell, human U20S whole cell, human MCF-7 whole cell, rat thymus tissue, rat PC-12 whole cell, mouse thymus tissue, mouse Raw2647 whole cell
FCM: MCF-7 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of EGR2 using anti-EGR2 antibody (A00921-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human U20S whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat thymus tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse thymus tissue lysates,
Lane 8: mouse Raw264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGR2 antigen affinity purified polyclonal antibody (Catalog # A00921-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGR2 at approximately 50 kDa. The expected band size for EGR2 is at 50,45 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-EGR2 antibody (A00921-1).
Overlay histogram showing MCF-7 cells stained with A00921-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EGR2 Antibody (A00921-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For EGR2 (Source: Uniprot.org, NCBI)
Gene Name
EGR2
Full Name
E3 SUMO-protein ligase EGR2
Weight
50,45 kDa
Superfamily
EGR C2H2-type zinc-finger protein family
Alternative Names
AT591; CMT1D; CMT4E; early growth response 2 (Krox-20 homolog, Drosophila); early growth response 2; early growth response protein 2; EGR-2; FLJ14547; Krox-20 homolog, Drosophila; KROX-20, Drosophila, homolog (early growth response-2); KROX20DKFZp686J1957; Zinc finger protein Krox-20 EGR2 AT591, CHN1, CMT1D, CMT4E, KROX20 early growth response 2 E3 SUMO-protein ligase EGR2|E3 SUMO-protein transferase ERG2|KROX-20, Drosophila, homolog (early growth response-2)|early growth response protein 2|zinc finger protein Krox-20
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EGR2, check out the EGR2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EGR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EGR2 Antibody Picoband® (A00921-1)
Hello CJ!
No publications found for A00921-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-EGR2 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-EGR2 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
