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Product Info Summary
| SKU: | M03755-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-EDG3 Rabbit Monoclonal Antibody
View all S1P3/EDG-3 Antibodies
SKU/Catalog Number
M03755-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-EDG3 Rabbit Monoclonal Antibody catalog # M03755-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EDG3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03755-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
25S21
Isotype
IgG
Immunogen
A synthesized peptide derived from human EDG3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03755-1 is reactive to S1PR3 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
42 kDa
Calculated molecular weight
30382 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03755-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: human HepG2 whole cell, human Hela whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of EDG3 using anti-EDG3 antibody (M03755-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EDG3 antigen affinity purified monoclonal antibody (Catalog # M03755-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EDG3 at approximately 42 kDa. The expected band size for EDG3 is at 42 kDa.
Protein Target Info & Infographic
Gene/Protein Information For S1PR3 (Source: Uniprot.org, NCBI)
Gene Name
S1PR3
Full Name
Sphingosine 1-phosphate receptor 3
Weight
30382 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
5'-AMP-activated protein kinase subunit beta-1;AMPK subunit beta-1;AMPKb;PRKAB1;AMPK; S1PR3 EDG-3, EDG3, LPB3, S1P3 sphingosine-1-phosphate receptor 3 sphingosine 1-phosphate receptor 3|G protein-coupled receptor, endothelial differentiation gene-3|S1P receptor 3|S1P receptor EDG3|S1P receptor Edg-3|endothelial differentiation G-protein coupled receptor 3|endothelial differentiation, sphingolipid G-protein-coupled receptor, 3|sphingosine 1-phosphate receptor Edg-3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on S1PR3, check out the S1PR3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for S1PR3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EDG3 Rabbit Monoclonal Antibody (M03755-1)
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