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Product Info Summary
| SKU: | M01920 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-E2F4 Monoclonal Antibody
SKU/Catalog Number
M01920
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-E2F4 Monoclonal Antibody catalog # M01920. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-E2F4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01920)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFDF-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human E2F4 Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01920 is reactive to E2F4 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
85 kDa
Calculated molecular weight
84640 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01920 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: Raji cell
Validation Images & Assay Conditions
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Western blot analysis of E2F4 expression in Raji cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For E2F4 (Source: Uniprot.org, NCBI)
Gene Name
E2F4
Full Name
Transcription factor E2F4
Weight
84640 MW
Superfamily
E2F/DP family
Alternative Names
Inhibitor of nuclear factor kappa-B kinase subunit alpha;I-kappa-B kinase alpha;IKK-A;IKK-alpha;IkBKA;IkappaB kinase;2.7.11.10;Conserved helix-loop-helix ubiquitous kinase;I-kappa-B kinase 1;IKK1;Nuclear factor NF-kappa-B inhibitor kinase alpha;NFKBIKA;Transcription factor 16;TCF-16;CHUK;IKKA, TCF16; E2F4 E2F-4 E2F transcription factor 4 transcription factor E2F4|E2F transcription factor 4, p107/p130-binding|p107/p130-binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on E2F4, check out the E2F4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for E2F4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-E2F4 Monoclonal Antibody (M01920)
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No publications found for M01920
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Customer Q&As
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1 Customer Q&As for Anti-E2F4 Monoclonal Antibody
Question
What sample was tested in WB and IHC applications with M01920?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of E2F4 expression in Raji cell lysate.
Boster Scientific Support
Answered: 2020-01-09
