Anti-Dyskerin DKC1 Antibody

Product Name

Anti-Dyskerin DKC1 Antibody

View all DKC1 Antibodies

SKU/Catalog Number

A01535-1

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Dyskerin DKC1 Antibody (Catalog # A01535-1). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Dyskerin DKC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01535-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from Internal of human Dyskerin.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01535-1 is reactive to DKC1 in Human, Mouse, Rat

Reconstitution

Calculated molecular weight

57674 MW

Background of DKC1

Isoform 1:Required for ribosome biogenesis and telomere maintenance. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.Isoform 3:Promotes cell to cell and cell to substratum adhesion, increases the cell proliferation rate and leads to cytokeratin hyper-expression (when overexpressed in HeLa cells).

Heiss N.S., Nat. Genet. 19:32-38(1998).
Knight S.W., Am. J. Hum. Genet. 65:50-58(1999).
Hassock S., Genomics 55:21-27(1999).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01535-1 is guaranteed for IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

Gene/Protein Information For DKC1 (Source: Uniprot.org, NCBI)

Alternative Names

H/ACA ribonucleoprotein complex subunit 4;5.4.99.-;CBF5 homolog;Dyskerin;Nopp140-associated protein of 57 kDa;Nucleolar protein NAP57;Nucleolar protein family A member 4;snoRNP protein DKC1;DKC1;NOLA4; DKC1 CBF5, DKC, DKCX, NAP57, NOLA4, XAP101 dyskerin pseudouridine synthase 1 H/ACA ribonucleoprotein complex subunit DKC1|CBF5 homolog|H/ACA ribonucleoprotein complex subunit 4|dyskeratosis congenita 1, dyskerin|nopp140-associated protein of 57 kDa|nucleolar protein NAP57|nucleolar protein family A member 4|snoRNP protein DKC1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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