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Product Info Summary
| SKU: | A03369-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-FOXN1 Antibody Picoband®
SKU/Catalog Number
A03369-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FOXN1 Antibody Picoband® catalog # A03369-2. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FOXN1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03369-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human FOXN1, which shares 85.7% amino acid (aa) sequence identity with mouse FOXN1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03369-2 is reactive to FOXN1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
69 kDa
Calculated molecular weight
68.925kDa
Background of FOXN1
The FOXN1 gene encodes a transcription factor that regulates the development of epithelial cells in the thymus and skin. FOXN1 is expressed in thymic epithelial cell precursors which interact with T-cell progenitors for T-cell development in the thymus, and thus plays an essential role in thymic development beginning in the embryo. FOXN1 is also expressed in keratinocytes, hair follicles, epithelial cells in the nail matrix bed, and cells of the choroid plexus.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03369-2 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human Hela whole cell, human Raji whole cell, mouse NIH/3T3 whole cell, mouse RAW2647 whole cell
FCM: SiHa cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FOXN1 using anti-FOXN1 antibody (A03369-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: mouse NIH/3T3 whole cell lysates,
Lane 4: mouse RAW264.7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXN1 antigen affinity purified polyclonal antibody (Catalog # A03369-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXN1 at approximately 69KD. The expected band size for FOXN1 is at 69KD.
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Figure 2. Flow Cytometry analysis of SiHa cells using anti-FOXN1 antibody (A03369-2).
Overlay histogram showing SiHa cells stained with A03369-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FOXN1 Antibody (A03369-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For FOXN1 (Source: Uniprot.org, NCBI)
Gene Name
FOXN1
Full Name
Forkhead box protein N1
Weight
68.925kDa
Alternative Names
Vitamin D-binding protein; DBP; VDB; Gc-globulin; Group-specific component; Gc FOXN1 FKHL20, RONU, TIDAND, TLIND, WHN forkhead box N1 forkhead box protein N1|Rowett nude|winged-helix nude|winged-helix transcription factor nude
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FOXN1, check out the FOXN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FOXN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FOXN1 Antibody Picoband® (A03369-2)
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