Click image to see more details
-
-
-
-
-
+1
Product Info Summary
| SKU: | M03454-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-DYNLL1/PIN Antibody Picoband® (monoclonal, 6G2H1)
SKU/Catalog Number
M03454-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DYNLL1/PIN Antibody Picoband® (monoclonal, 6G2H1) catalog # M03454-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-DYNLL1/PIN Antibody Picoband® (monoclonal, 6G2H1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03454-1)
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
6G2H1
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human DYNLL1/PIN recombinant protein (Position: M1-G89).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M03454-1 is reactive to DYNLL1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
12 kDa
Calculated molecular weight
10.366kDa
Background of PIN/DLC8
Dynein light chain 1, cytoplasmic is a protein that in humans is encoded by the DYNLL1 gene. Cytoplasmic dyneins are large enzyme complexes with a molecular mass of about 1,200 kD. They contain two force-producing heads formed primarily from dynein heavy chains, and stalks linking the heads to a basal domain, which contains a varying number of accessory intermediate chains. The complex is involved in intracellular transport and motility. The protein described in this record is a light chain and exists as part of this complex but also physically interacts with and inhibits the activity of neuronal nitric oxide synthase. Binding of this protein destabilizes the neuronal nitric oxide synthase dimer, a conformation necessary for activity, and it may regulate numerous biologic processes through its effects on nitric oxide synthase activity. Alternate transcriptional splice variants have been characterized.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03454-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human HepG2 whole cell, human PC-3 whole cell, human U87 whole cell, human MCF-7 whole cell, rat testis tissue, rat brain tissue, mouse testis tissue, mouse brain tissue
IHC: human bladder epithelial carcinoma tissue, human metaplasia of squamous cells of the renal pelvis tissue
ICC/IF: MCF-7 cell
FCM: SiHa cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of DYNLL1/PIN using anti-DYNLL1/PIN antibody (M03454-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human U87 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse testis tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-DYNLL1/PIN antigen affinity purified monoclonal antibody (Catalog # M03454-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for DYNLL1/PIN at approximately 12 kDa. The expected band size for DYNLL1/PIN is at 12 kDa.
Click image to see more details
Figure 2. IHC analysis of DYNLL1/PIN using anti-DYNLL1/PIN antibody (M03454-1).
DYNLL1/PIN was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-DYNLL1/PIN Antibody (M03454-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of DYNLL1/PIN using anti-DYNLL1/PIN antibody (M03454-1).
DYNLL1/PIN was detected in a paraffin-embedded section of human metaplasia of squamous cells of the renal pelvis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-DYNLL1/PIN Antibody (M03454-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 4. IF analysis of DYNLL1/PIN using anti-DYNLL1/PIN antibody (M00179-1).
DYNLL1/PIN was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-DYNLL1/PIN Antibody (M00179-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 5. Flow Cytometry analysis of SiHa cells using anti-DYNLL1/PIN antibody (M03454-1).
Overlay histogram showing SiHa cells stained with M03454-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-DYNLL1/PIN Antibody (M03454-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For DYNLL1 (Source: Uniprot.org, NCBI)
Gene Name
DYNLL1
Full Name
Dynein light chain 1, cytoplasmic
Weight
10.366kDa
Superfamily
dynein light chain family
Alternative Names
Survival motor neuron protein; Component of gems 1; Gemin-1; SMN1; SMN, SMNT; SMN2; SMNC DYNLL1 DLC1, DLC8, DNCL1, DNCLC1, LC8, LC8a, PIN, hdlc1 dynein light chain LC8-type 1 dynein light chain 1, cytoplasmic|8 kDa dynein light chain|cytoplasmic dynein light polypeptide|dynein, cytoplasmic, light polypeptide 1|protein inhibitor of neuronal nitric oxide synthase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DYNLL1, check out the DYNLL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DYNLL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DYNLL1/PIN Antibody Picoband® (monoclonal, 6G2H1) (M03454-1)
Hello CJ!
No publications found for M03454-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-DYNLL1/PIN Antibody Picoband® (monoclonal, 6G2H1)?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-DYNLL1/PIN Antibody Picoband® (monoclonal, 6G2H1)
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
