Click image to see more details
Product Info Summary
| SKU: | M02536-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Dynamin 1 DNM1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02536-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Dynamin 1 DNM1 Rabbit Monoclonal Antibody catalog # M02536-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Dynamin 1 DNM1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02536-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACHH-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Dynamin 1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02536-1 is reactive to DNM1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
52 kDa
Calculated molecular weight
119700 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02536-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: SH-SY5Y cell, NIH/3T3 cell
Validation Images & Assay Conditions
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Western blot analysis of Dynamin 1 expression in (1) SH-SY5Y cell lysate; (2) NIH/3T3 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For DNM1 (Source: Uniprot.org, NCBI)
Gene Name
DNM1
Full Name
Dynamin-1
Weight
119700 MW
Superfamily
TRAFAC class dynamin-like GTPase superfamily
Alternative Names
Proline-, glutamic acid- and leucine-rich protein 1;Modulator of non-genomic activity of estrogen receptor;Transcription factor HMX3;PELP1;HMX3, MNAR; DNM1 DEE31, DNM, EIEE31 dynamin 1 dynamin-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DNM1, check out the DNM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DNM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Dynamin 1 DNM1 Rabbit Monoclonal Antibody (M02536-1)
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No publications found for M02536-1
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Customer Q&As
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16 Customer Q&As for Anti-Dynamin 1 DNM1 Rabbit Monoclonal Antibody
Question
Our lab were satisfied with the WB result of your anti-Dynamin 1 Monoclonal antibody. However we have been able to see positive staining in brain pns cytoplasm. using this antibody. Is that expected? Could you tell me where is DNM1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
According to literature, brain pns does express DNM1. Generally DNM1 expresses in cytoplasm. Regarding which tissues have DNM1 expression, here are a few articles citing expression in various tissues:
Brain, and PNS, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2020-04-16
Question
What sample target on Western Blot application was tested with M02536-1?
Verified customer
Asked: 2020-01-03
Answer
Western blot analysis of Dynamin 1 expression in (1) SH-SY5Y cell lysate; (2) NIH/3T3 cell lysate.
Boster Scientific Support
Answered: 2020-01-16
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Dynamin 1 Monoclonal antibody M02536-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-31
Question
Would M02536-1 anti-Dynamin 1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
It shows on the product datasheet, M02536-1 anti-Dynamin 1 Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-28
Question
Is there a BSA free version of anti-Dynamin 1 Monoclonal antibody M02536-1 available?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Dynamin 1 Monoclonal antibody M02536-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-25
Question
Is this M02536-1 anti-Dynamin 1 Monoclonal antibody reactive to the isotypes of DNM1?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
The immunogen of M02536-1 anti-Dynamin 1 Monoclonal antibody is A synthesized peptide derived from human Dynamin 1 Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Involved in receptor-mediated endocytosis. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-20
Question
We have been able to see staining in human brain pns. Any tips? Is anti-Dynamin 1 Monoclonal antibody supposed to stain brain pns positively?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
From what I have seen in literature brain pns does express DNM1. From what I have seen in Uniprot.org, DNM1 is expressed in frontal cortex, brain pns, cervix carcinoma, platelet, among other tissues. Regarding which tissues have DNM1 expression, here are a few articles citing expression in various tissues:
Brain, and PNS, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2019-05-20
Question
I see that the anti-Dynamin 1 Monoclonal antibody M02536-1 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-01-28
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-01-28
Question
I was wanting to use your anti-Dynamin 1 Monoclonal antibody for IF for mouse cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2018-09-06
Answer
As indicated on the product datasheet, M02536-1 anti-Dynamin 1 Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-06
Question
My question regarding product M02536-1, anti-Dynamin 1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Huang
Verified customer
Asked: 2018-03-30
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02536-1 anti-Dynamin 1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-03-30
Question
you antibody to test anti-Dynamin 1 Monoclonal antibody M02536-1 on mouse cervix carcinoma for research purposes, then I may be interested in using anti-Dynamin 1 Monoclonal antibody M02536-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-02-09
Answer
The products we sell, including anti-Dynamin 1 Monoclonal antibody M02536-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-02-09
Question
Does anti-Dynamin 1 Monoclonal antibody M02536-1 work for IF with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2018-02-01
Answer
According to the expression profile of cervix carcinoma, DNM1 is highly expressed in cervix carcinoma. So, it is likely that anti-Dynamin 1 Monoclonal antibody M02536-1 will work for IF with cervix carcinoma.
Boster Scientific Support
Answered: 2018-02-01
Question
See attached the WB image, lot number and protocol we used for cervix carcinoma using anti-Dynamin 1 Monoclonal antibody M02536-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-09-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-26
Question
We are currently using anti-Dynamin 1 Monoclonal antibody M02536-1 for rat tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
J. Carter
Verified customer
Asked: 2016-01-18
Answer
The anti-Dynamin 1 Monoclonal antibody (M02536-1) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-01-18
Question
We ordered your anti-Dynamin 1 Monoclonal antibody for WB on platelet in the past. I am using mouse, and We are going to use the antibody for IF next. My lab would like examining platelet as well as brain pns in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
E. Carter
Verified customer
Asked: 2014-05-13
Answer
I have checked the website and datasheets of our anti-Dynamin 1 Monoclonal antibody and it seems that M02536-1 has been validated on mouse in both WB and IF. Thus M02536-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-05-13
Question
Is a blocking peptide available for product anti-Dynamin 1 Monoclonal antibody (M02536-1)?
S. Li
Verified customer
Asked: 2013-05-29
Answer
We do provide the blocking peptide for product anti-Dynamin 1 Monoclonal antibody (M02536-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-05-29
