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Product Info Summary
| SKU: | A01681 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-EPS15 Antibody Picoband®
SKU/Catalog Number
A01681
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-EPS15 Antibody Picoband® catalog # A01681. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EPS15 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01681)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human EPS15 recombinant protein (Position: A2-E88).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01681 is reactive to EPS15 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
140 kDa
Calculated molecular weight
50514 MW
Background of EPS15
Epidermal growth factor receptor substrate 15 is a protein that in humans is encoded by the EPS15 gene. This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01681 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human A375 whole cell, human HepG2 whole cell, human Jurkat whole cell, human PANC-1 whole cell, rat brain tissue, rat liver tissue, mouse brain tissue, mouse spleen tissue, mouse liver tissue, mouse testis tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of EPS15 using anti-EPS15 antibody (A01681).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A375 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human PANC-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPS15 antigen affinity purified polyclonal antibody (Catalog # A01681) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPS15 at approximately 140KD. The expected band size for EPS15 is at 99KD.
Click image to see more details
Figure 2. Western blot analysis of EPS15 using anti-EPS15 antibody (A01681).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse spleen tissue lysates,
Lane 5: mouse liver tissue lysates,
Lane 6: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPS15 antigen affinity purified polyclonal antibody (Catalog # A01681) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPS15 at approximately 140KD. The expected band size for EPS15 is at 99KD.
Protein Target Info & Infographic
Gene/Protein Information For EPS15 (Source: Uniprot.org, NCBI)
Gene Name
EPS15
Full Name
Epidermal growth factor receptor substrate 15
Weight
50514 MW
Alternative Names
Epidermal growth factor receptor substrate 15; Protein Eps15; Protein AF-1p; EPS15; AF1P EPS15 AF-1P, AF1P, MLLT5 epidermal growth factor receptor pathway substrate 15 epidermal growth factor receptor substrate 15|ALL1 fused gene from chromosome 1|protein AF-1p
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EPS15, check out the EPS15 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EPS15: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EPS15 Antibody Picoband® (A01681)
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1 Customer Q&As for Anti-EPS15 Antibody Picoband®
Question
We are currently using anti-EPS15 antibody A01681 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
The anti-EPS15 antibody (A01681) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-23
