Product Info Summary
SKU: | PA1762 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-DUSP3 Antibody
SKU/Catalog Number
PA1762
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DUSP3 Antibody catalog # PA1762. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DUSP3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1762)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human DUSP3, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1762 is reactive to DUSP3 in Human, Mouse, Rat
Applications
PA1762 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
11 kDa
Calculated molecular weight
20.478kDa
Background of VHR/DUSP3
DUSP3 (Dual-specificity phosphatase 3), also called VHR, is a member of the dual specificity protein phosphatase subfamily. DUSPs constitute a large heterogeneous subgroup of the type I cysteine-based protein-tyrosine phosphatase superfamily. DUSPs are characterized by their ability to dephosphorylate both tyrosine and serine/threonine residues. DUSP3 contains the consensus DUSP C-terminal catalytic domain but lacks the N-terminal CH2 domain found in the MKP (mitogen-activated protein kinase phosphatase) class of DUSPs. The DUSP3 gene is mapped on 17q21.31. Confocal microscopy demonstrated that phosphorylated VHR accumulated at the immune synapse between the T cell and the antigen-presenting cell in the presence of antigen. Tyrosine phosphorylation of VHR affects protein-protein interaction, subcellular location, or substrate targeting, given that tyr138 is located on the opposite side of the VHR catalytic center.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Validation Images & Assay Conditions
Click image to see more details
Anti-DUSP3 antibody, PA1762, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: SKOV Cell Lysate
Lane 3: MM453 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For DUSP3 (Source: Uniprot.org, NCBI)
Gene Name
DUSP3
Full Name
Dual specificity protein phosphatase 3
Weight
20.478kDa
Superfamily
protein-tyrosine phosphatase family
Alternative Names
dual specificity phosphatase 3; dual specificity protein phosphatase 3; Dual specificity protein phosphatase VHR; DUSP3; EC 3.1.3.16; EC 3.1.3.48; Vaccinia H1-related phosphatase; vaccinia virus phosphatase VH1-related; VHR; VHRserine/threonine specific protein phosphatase DUSP3 VHR dual specificity phosphatase 3 dual specificity protein phosphatase 3|dual specificity protein phosphatase VHR|serine/threonine specific protein phosphatase|vaccinia H1-related phosphatase|vaccinia virus phosphatase VH1-related
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DUSP3, check out the DUSP3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DUSP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DUSP3 Antibody (PA1762)
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No publications found for PA1762
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-DUSP3 Antibody
Question
Would PA1762 anti-DUSP3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Gonzalez
Verified customer
Asked: 2019-12-20
Answer
It shows on the product datasheet, PA1762 anti-DUSP3 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-20
Question
We are currently using anti-DUSP3 antibody PA1762 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
E. Banerjee
Verified customer
Asked: 2019-09-10
Answer
The anti-DUSP3 antibody (PA1762) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-10
Question
Will anti-DUSP3 antibody PA1762 work on canine WB with duodenum uterus?
J. Huang
Verified customer
Asked: 2015-03-24
Answer
Our lab technicians have not validated anti-DUSP3 antibody PA1762 on canine. You can run a BLAST between canine and the immunogen sequence of anti-DUSP3 antibody PA1762 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine duodenum uterus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-03-24
Question
Is a blocking peptide available for product anti-DUSP3 antibody (PA1762)?
P. Singh
Verified customer
Asked: 2013-05-24
Answer
We do provide the blocking peptide for product anti-DUSP3 antibody (PA1762). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-05-24