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Product Info Summary
| SKU: | M01053-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Doublecortin/DCX Monoclonal Antibody
View all Doublecortin Antibodies
SKU/Catalog Number
M01053-1
Size
100ul
Description
Boster Bio Anti-Doublecortin/DCX Monoclonal Antibody catalog # M01053-1. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Doublecortin/DCX Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01053-1)
Host
Mouse
Contents
Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Clonality
Monoclonal
Immunogen
Recombinant full length human Lis-A isoform of DCX purified from E. coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01053-1 is reactive to DCX in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
40574 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01053-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:10,000. IF/ICC and IHC: 1:1,000.
Validation Images & Assay Conditions
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Western blot analysis of rat whole brain lysates using mouse mAb to doublecortin (DCX), M01053-1, dilution 1:1,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain. Strong bands at 40kDa and 45kDa correspond to the DCX protein, detected only in the developing brain.
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Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to doublecortin (DCX), M01053-1, dilution 1:1,000 in red, and costained with chicken pAb to microtubule associated protein 2 (MAP2), dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA. DCX antibody reveals strong cytoplasmic staining in a population of small developing neurons and their processes, while the MAP2 antibody stains dendrites and perikarya of mature neurons. The DCX antibody is an excellent marker of early developing neuronal cells.
Protein Target Info & Infographic
Gene/Protein Information For DCX (Source: Uniprot.org, NCBI)
Gene Name
DCX
Full Name
Neuronal migration protein doublecortin
Weight
40574 MW
Alternative Names
Neuronal migration protein doublecortin;Doublin;Lissencephalin-X;Lis-X;DCX;DBCN, LISX; DCX DBCN, DC, LISX, SCLH, XLIS doublecortin neuronal migration protein doublecortin|doublecortex|doublin|lis-X|lissencephalin-X
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DCX, check out the DCX Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DCX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Doublecortin/DCX Monoclonal Antibody (M01053-1)
Hello CJ!
M01053-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang Xm, Du F, Yang D, Yu Cj, Huang Xn, Liu W, Fu J. Bmc Neurosci. 2010 Oct 25;11:138. Doi: 10.1186/1471-2202-11-138. Transplanted Bone Marrow Stem Cells Relocate To Infarct Penumbra And Co-Express Endogenous Proliferative And Immature Neuronal M...
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Customer Q&As
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17 Customer Q&As for Anti-Doublecortin/DCX Monoclonal Antibody
Question
At what pH are you recommending to use for target retrieval for IHC using M01053-1?
Verified customer
Asked: 2022-02-21
Answer
When we do antigen retrieval using Anti-Doublecortin/DCX Monoclonal Antibody (M01053-1), the buffer is pH 6.0.
Boster Scientific Support
Answered: 2022-03-04
Question
My question regarding product M01053-1, anti-Doublecortin/DCX Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01053-1 anti-Doublecortin/DCX Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-25
Question
I see that the anti-Doublecortin/DCX Monoclonal antibody M01053-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-29
Question
Is a blocking peptide available for product anti-Doublecortin/DCX Monoclonal antibody (M01053-1)?
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
We do provide the blocking peptide for product anti-Doublecortin/DCX Monoclonal antibody (M01053-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-01
Question
We are currently using anti-Doublecortin/DCX Monoclonal antibody M01053-1 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
The anti-Doublecortin/DCX Monoclonal antibody (M01053-1) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-29
Question
you antibody using your anti-Doublecortin/DCX Monoclonal antibody for x-linked 1 (lisx1) studies. Has this antibody been tested with western blotting on developing neuronal cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
I appreciate your inquiry. This M01053-1 anti-Doublecortin/DCX Monoclonal antibody is tested on developing neuronal cells. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-20
Question
Will anti-Doublecortin/DCX Monoclonal antibody M01053-1 work for WB with brain?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
According to the expression profile of brain, DCX is highly expressed in brain. So, it is likely that anti-Doublecortin/DCX Monoclonal antibody M01053-1 will work for WB with brain.
Boster Scientific Support
Answered: 2019-08-05
Question
Our lab want to know about to test anti-Doublecortin/DCX Monoclonal antibody M01053-1 on mouse brain for research purposes, then I may be interested in using anti-Doublecortin/DCX Monoclonal antibody M01053-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
The products we sell, including anti-Doublecortin/DCX Monoclonal antibody M01053-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-03
Question
Is this M01053-1 anti-Doublecortin/DCX Monoclonal antibody reactive to the isotypes of DCX?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
The immunogen of M01053-1 anti-Doublecortin/DCX Monoclonal antibody is Recombinant full length human Lis-A isoform of DCX purified from E. coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-29
Question
Please see the WB image, lot number and protocol we used for brain using anti-Doublecortin/DCX Monoclonal antibody M01053-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-17
Question
My colleagues were content with the WB result of your anti-Doublecortin/DCX Monoclonal antibody. However we have been able to see positive staining in brain cytoplasm. cell projection, using this antibody. Is that expected? Could you tell me where is DCX supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-03-11
Answer
From literature, brain does express DCX. Generally DCX expresses in cytoplasm. cell projection,. Regarding which tissues have DCX expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9489700, 15489334
Cervix carcinoma, Pubmed ID: 17081983
Fetal brain, Pubmed ID: 9489699, 15772651
Boster Scientific Support
Answered: 2019-03-11
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Doublecortin/DCX Monoclonal antibody M01053-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-11-09
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-09
Question
I was wanting to use your anti-Doublecortin/DCX Monoclonal antibody for WB for mouse brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain identification?
Verified Customer
Verified customer
Asked: 2018-11-08
Answer
You can see on the product datasheet, M01053-1 anti-Doublecortin/DCX Monoclonal antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-08
Question
Is there a BSA free version of anti-Doublecortin/DCX Monoclonal antibody M01053-1 available?
Verified Customer
Verified customer
Asked: 2018-06-18
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Doublecortin/DCX Monoclonal antibody M01053-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-06-18
Question
We bought anti-Doublecortin/DCX Monoclonal antibody for WB on fetal brain a few years ago. I am using rat, and We want to use the antibody for IHC next. I would like examining fetal brain as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2017-06-28
Answer
I looked at the website and datasheets of our anti-Doublecortin/DCX Monoclonal antibody and it seems that M01053-1 has been tested on rat in both WB and IHC. Thus M01053-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-06-28
Question
Does M01053-1 anti-Doublecortin/DCX Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Baker
Verified customer
Asked: 2014-11-20
Answer
As indicated on the product datasheet, M01053-1 anti-Doublecortin/DCX Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-11-20
Question
We have seen staining in rat brain. What should we do? Is anti-Doublecortin/DCX Monoclonal antibody supposed to stain brain positively?
M. Dhar
Verified customer
Asked: 2013-08-02
Answer
From what I have seen in literature brain does express DCX. From what I have seen in Uniprot.org, DCX is expressed in frontal cortex, fetal brain, brain, cervix carcinoma, among other tissues. Regarding which tissues have DCX expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9489700, 15489334
Cervix carcinoma, Pubmed ID: 17081983
Fetal brain, Pubmed ID: 9489699, 15772651
Boster Scientific Support
Answered: 2013-08-02
