Click image to see more details
Product Info Summary
| SKU: | M01053 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-DCX/Doublecortin Rabbit Monoclonal Antibody
View all Doublecortin Antibodies
SKU/Catalog Number
M01053
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-DCX/Doublecortin Rabbit Monoclonal Antibody catalog # M01053. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DCX/Doublecortin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01053)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOAD-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human DCX
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01053 is reactive to DCX in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
45 kDa
Calculated molecular weight
40574 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01053 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
FC 1:50-1:200
Positive Control
WB: C6 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of DCX expression in C6 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For DCX (Source: Uniprot.org, NCBI)
Gene Name
DCX
Full Name
Neuronal migration protein doublecortin
Weight
40574 MW
Alternative Names
Neuronal migration protein doublecortin;Doublin;Lissencephalin-X;Lis-X;DCX;DBCN, LISX; DCX DBCN, DC, LISX, SCLH, XLIS doublecortin neuronal migration protein doublecortin|doublecortex|doublin|lis-X|lissencephalin-X
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DCX, check out the DCX Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DCX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DCX/Doublecortin Rabbit Monoclonal Antibody (M01053)
Hello CJ!
M01053 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang Xm, Du F, Yang D, Wang R, Yu Cj, Huang Xn, Hu Hy, Liu W, Fu J. Bmc Neurosci. 2011 Jun 24;12:61. Doi: 10.1186/1471-2202-12-61. Granulocyte Colony-Stimulating Factor Increases The Therapeutic Efficacy Of Bone Marrow Mononuclear Cell Transplant...
Zhang Xm, Du F, Yang D, Yu Cj, Huang Xn, Liu W, Fu J. Bmc Neurosci. 2010 Oct 25;11:138. Doi: 10.1186/1471-2202-11-138. Transplanted Bone Marrow Stem Cells Relocate To Infarct Penumbra And Co-Express Endogenous Proliferative And Immature Neuronal M...
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-DCX/Doublecortin Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-DCX/Doublecortin Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
3 Customer Q&As for Anti-DCX/Doublecortin Rabbit Monoclonal Antibody
Question
We are currently using anti-DCX/Doublecortin Rabbit Monoclonal antibody M01053 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
L. Miller
Verified customer
Asked: 2020-03-02
Answer
The anti-DCX/Doublecortin Rabbit Monoclonal antibody (M01053) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-02
Question
My team were content with the WB result of your anti-DCX/Doublecortin Rabbit Monoclonal antibody. However we have been able to see positive staining in cervix carcinoma cytoplasm. cell projection, using this antibody. Is that expected? Could you tell me where is DCX supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
From what I have seen in literature, cervix carcinoma does express DCX. Generally DCX expresses in cytoplasm. cell projection,. Regarding which tissues have DCX expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9489700, 15489334
Cervix carcinoma, Pubmed ID: 17081983
Fetal brain, Pubmed ID: 9489699, 15772651
Boster Scientific Support
Answered: 2019-11-01
Question
We have observed staining in human cervix carcinoma. What should we do? Is anti-DCX/Doublecortin Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2017-06-06
Answer
From what I have seen in literature cervix carcinoma does express DCX. From what I have seen in Uniprot.org, DCX is expressed in frontal cortex, fetal brain, brain, cervix carcinoma, among other tissues. Regarding which tissues have DCX expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9489700, 15489334
Cervix carcinoma, Pubmed ID: 17081983
Fetal brain, Pubmed ID: 9489699, 15772651
Boster Scientific Support
Answered: 2017-06-06
