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Product Info Summary
| SKU: | A00369-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Dopamine Transporter/SLC6A3 Antibody Picoband®
View all SLC6A3/DAT1 Antibodies
SKU/Catalog Number
A00369-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Dopamine Transporter/SLC6A3 Antibody Picoband® catalog # A00369-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Dopamine Transporter/SLC6A3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00369-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Dopamine Transporter/SLC6A3 recombinant protein (Position: M1-V620).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00369-3 is reactive to SLC6A3 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
68 kDa
Calculated molecular weight
131117 MW
Background of SLC6A3/DAT1
This gene encodes a dopamine transporter which is a member of the sodium- and chloride-dependent neurotransmitter transporter family. The 3' UTR of this gene contains a 40 bp tandem repeat, referred to as a variable number tandem repeat or VNTR, which can be present in 3 to 11 copies. Variation in the number of repeats is associated with idiopathic epilepsy, attention-deficit hyperactivity disorder, dependence on alcohol and cocaine, susceptibility to Parkinson disease and protection against nicotine dependence.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00369-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human 293T whole cell, human MCF-7 whole cell, human HepG2 whole cell, human Caco-2 whole cell, human A431 whole cell
FCM: THP-1 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Dopamine Transporter/SLC6A3 using anti-Dopamine Transporter/SLC6A3 antibody (A00369-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dopamine Transporter/SLC6A3 antigen affinity purified polyclonal antibody (Catalog # A00369-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dopamine Transporter/SLC6A3 at approximately 68 kDa. The expected band size for Dopamine Transporter/SLC6A3 is at 68 kDa.
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Figure 2. Flow Cytometry analysis of THP-1 cells using anti-Dopamine Transporter/SLC6A3 antibody (A00369-3).
Overlay histogram showing THP-1 cells stained with A00369-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Dopamine Transporter/SLC6A3 Antibody (A00369-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For SLC6A3 (Source: Uniprot.org, NCBI)
Gene Name
SLC6A3
Full Name
Sodium-dependent dopamine transporter
Weight
131117 MW
Superfamily
sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family
Alternative Names
Nitric oxide synthase, inducible;1.14.13.39;Hepatocyte NOS;HEP-NOS;Inducible NO synthase;Inducible NOS;iNOS;N SLC6A3 DAT, DAT1, PKDYS, PKDYS1 solute carrier family 6 member 3 sodium-dependent dopamine transporter|DA transporter|dopamine transporter 1|solute carrier family 6 (neurotransmitter transporter), member 3|solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC6A3, check out the SLC6A3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC6A3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Dopamine Transporter/SLC6A3 Antibody Picoband® (A00369-3)
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