Anti-Phospho-Dopamine Transporter (DAT) (Thr53) Antibody

Product Name

Anti-Phospho-Dopamine Transporter (DAT) (Thr53) Antibody

View all SLC6A3/DAT1 Antibodies

SKU/Catalog Number

P00369

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-Dopamine Transporter (DAT) (Thr53) Antibody (Catalog # P00369). Tested in WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-Dopamine Transporter (DAT) (Thr53) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00369)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr53 of rat dopamine transporter, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P00369 is reactive to SLC6A3 in Mouse, Rat

Reconstitution

Observed Molecular Weight

52 kDa

Calculated molecular weight

68495 MW

Background of SLC6A3/DAT1

The dopamine transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996). It has been shown that phosphorylation at Thr-53 directly affects dopamine influx and amphetamine-stimulated substrate efflux, indicating that the Thr-53 residue plays a major role in transport activity (Foster et al., 2012).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

P00369 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

Gene/Protein Information For SLC6A3 (Source: Uniprot.org, NCBI)

Alternative Names

DA transporter antibody, DAT 1 antibody, DAT antibody, DAT1 antibody, Dopamine transporter 1 antibody, Dopamine transporter antibody, PKDYS antibody, SC6A3_HUMAN antibody, SLC6A3 antibody, Sodium dependent dopamine transporter antibody, Sodium-dependent dopamine transporter antibody, Solute carrier family 6 (neurotransmitter transporter dopamine) member 3 antibody, Solute carrier family 6 (neurotransmitter transporter) member 3 antibody, Solute carrier family 6 member 3 antibody, Variable number tandem repeat (VNTR) antibody SLC6A3 DAT, DAT1, PKDYS, PKDYS1 solute carrier family 6 member 3 sodium-dependent dopamine transporter|DA transporter|dopamine transporter 1|solute carrier family 6 (neurotransmitter transporter), member 3|solute carrier family 6 (neurotransmitter transporter, dopamine), member 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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