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Product Info Summary
| SKU: | EKC2316 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human, Mouse, Rat |
| Application: | ELISA |
| Sample Types: | Cell lines |
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Product info
Product Name
eNOS (Phospho-Ser615) Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC2316
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The eNOS (Phospho-Ser615) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor eNOS (Phospho-Ser615) protein expression profile in cells. The kit can be used for measuring the relative amounts of eNOS (Phospho-Ser615) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on eNOS (Phospho-Ser615).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
eNOS (Phospho-Ser615) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2316)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2316 is reactive to NOS3 in Human, Mouse, Rat samples
Validated Sample Types
Cell lines
Application Guarantee
EKC2316 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-eNOS Phospho-Ser615 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-eNOS Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from K562 cells treated with EGF 40nM 30'
Protein Target Info & Infographic
Gene/Protein Information For NOS3 (Source: Uniprot.Org, NCBI)
Gene Name
NOS3
Full Name
Nitric oxide synthase, endothelial
Weight
133289 MW
Superfamily
NOS family
Alternative Names
cNOS;Constitutive NOS;EC-NOS;ECNOS;Endothelial NOS;Nitric-oxide synthase, endothelial;NOS, type III;NOS3;NOSIII NOS3 ECNOS, eNOS nitric oxide synthase 3 nitric oxide synthase, endothelial|EC-NOS|NOS type III|NOSIII|cNOS|constitutive NOS|endothelial NOS|nitric oxide synthase 3 (endothelial cell)|nitric oxide synthase 3 transcript variant eNOS-delta20|nitric oxide synthase 3 transcript variant eNOS-delta20-21|nitric oxide synthase 3 transcript variant eNOS-delta21
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on NOS3, check out the NOS3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for NOS3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For eNOS (Phospho-Ser615) Colorimetric Cell-Based ELISA Kit (EKC2316)
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