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Product Info Summary
| SKU: | A12371 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Dynein light chain 1, axonemal DNAL1 Antibody
SKU/Catalog Number
A12371
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Dynein light chain 1, axonemal DNAL1 Antibody (Catalog # A12371). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Dynein light chain 1, axonemal DNAL1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12371)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human DNAL1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12371 is reactive to DNAL1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
21533 MW
Background of DNAL1
This gene encodes an axonemal dynein light chain which functions as a component of the outer dynein arms complex. This complex acts as the molecular motor that provides the force to move cilia in an ATP-dependent manner. The encoded protein is expressed in tissues with motile cilia or flagella and may be involved in the movement of sperm flagella. Alternate splicing results in multiple transcript variants.
Horvath J., Am. J. Respir. Cell Mol. Biol. 33:41-47(2005).
The German cDNA consortium; Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12371 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from HepG2 cells, HeLa cells and MCF-7cells, using DNAL1 antibody A12371.
Protein Target Info & Infographic
Gene/Protein Information For DNAL1 (Source: Uniprot.org, NCBI)
Gene Name
DNAL1
Full Name
Dynein light chain 1, axonemal
Weight
21533 MW
Superfamily
dynein light chain LC1-type family
Alternative Names
Dynein light chain 1, axonemal;DNAL1;C14orf168; DNAL1 C14orf168, CILD16, LC1 dynein axonemal light chain 1 dynein light chain 1, axonemal
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DNAL1, check out the DNAL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DNAL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Dynein light chain 1, axonemal DNAL1 Antibody (A12371)
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No publications found for A12371
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Dynein light chain 1, axonemal DNAL1 Antibody
Question
Would A12371 anti-DNAL1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
You can see on the product datasheet, A12371 anti-DNAL1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-12
Question
We are currently using anti-DNAL1 antibody A12371 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
The anti-DNAL1 antibody (A12371) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-21
Question
I was wanting to use your anti-DNAL1 antibody for WB for human lymph node on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lymph node identification?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
As indicated on the product datasheet, A12371 anti-DNAL1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human lymph node in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-21
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymph node using anti-DNAL1 antibody A12371. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-12-29
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-29
Question
My question regarding product A12371, anti-DNAL1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-10-11
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A12371 anti-DNAL1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-11
Question
Is there a BSA free version of anti-DNAL1 antibody A12371 available?
Verified Customer
Verified customer
Asked: 2017-06-08
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-DNAL1 antibody A12371 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-06-08
