Product Info Summary
SKU: | A01376 |
---|---|
Size: | 100μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-DNA pol iota Antibody
View all DNA Polymerase iota Antibodies
SKU/Catalog Number
A01376
Size
100μl
Form
Liquid
Description
Boster Bio Anti-DNA pol iota Antibody catalog # A01376. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DNA pol iota Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01376)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01376 is reactive to POLI in Human
Applications
A01376 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
83006 MW
Background of DNA Polymerase iota
eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with eIF3, mutually exclusive with EIF4A1 or EIFA2, EIF4E and through its N-terminus with PAPBC1. Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA. Rapamycin can attenuate insulin stimulation mediated by FKBPs. Interacts with EIF4E3. Interacts with MIF4GD. Interacts with rotavirus A NSP3; in this interaction, NSP3 takes the place of PABPC1 thereby inducing shutoff of host protein synthesis
De Gregorio, E. et al. (1998) RNA 4, 828-836.
Ohlmann, T. et al. (1996) EMBO J. 15, 1371-1382.
Borman, A.M. and Kean, K.M. (1997) Virology 237, 129-136.
Gradi, A. et al. (1998) Mol Cell Biol 18, 334-42.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of HeLa cells using DNA pol iota Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For POLI (Source: Uniprot.org, NCBI)
Gene Name
POLI
Full Name
DNA polymerase iota
Weight
83006 MW
Superfamily
DNA polymerase type-Y family
Alternative Names
DNA polymerase iota; EC 2.7.7.7; eta2; polymerase (DNA directed) iota; RAD30 homolog B; RAD30Bpolymerase (DNA-directed), iota; RAD3OB POLI RAD30B, RAD3OB, eta2 DNA polymerase iota DNA polymerase iota|RAD30 homolog B|polymerase (DNA directed) iota|polymerase (DNA) iota|polymerase (DNA-directed), iota
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POLI, check out the POLI Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLI: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DNA pol iota Antibody (A01376)
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