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Product Info Summary
| SKU: | A00875-4 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-DLL4 Antibody Picoband®
SKU/Catalog Number
A00875-4
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-DLL4 Antibody Picoband® catalog # A00875-4. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-DLL4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00875-4)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human DLL4 recombinant protein (Position: S27-E678).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00875-4 is reactive to DLL4 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
80 kDa
Calculated molecular weight
48198 MW
Background of Dll4
Delta-like 4 is a protein that in humans is encoded by the DLL4 gene. It is mapped to 15q15.1. This gene is a homolog of the Drosophila delta gene. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. And this gene plays an important role in promoting Th17 effector activity during mycobacterial challenge.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00875-4 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human A431 whole cell, human 293T whole cell, human Caco-2 whole cell, human PC-3 whole cell, rat brain tissue, mouse brain tissue
FCM: HepG2 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of DLL4 using anti-DLL4 antibody (A00875-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLL4 antigen affinity purified polyclonal antibody (Catalog # A00875-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DLL4 at approximately 80 kDa. The expected band size for DLL4 is at 75 kDa.
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Figure 2. Flow Cytometry analysis of HepG2 cells using anti-DLL4 antibody (A00875-4).
Overlay histogram showing HepG2 cells stained with A00875-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-DLL4 Antibody (A00875-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For DLL4 (Source: Uniprot.org, NCBI)
Gene Name
DLL4
Full Name
Delta-like protein 4
Weight
48198 MW
Alternative Names
NF-kappa-B essential modulator;NEMO;FIP-3;IkB kinase-associated protein 1;IKKAP1;Inhibitor of nuclear factor kappa-B kinase subunit gamma;I-kappa-B kinase subunit gamma;IKK-gamma;IKKG;IkB kinase subunit gamma;NF-kappa-B essential modifier;IKBKG;FIP3, NEMO; Dll4|Delt, Delta4|delta like canonical Notch ligand 4|delta-like protein 4|delta-like 4|drosophila Delta homolog 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DLL4, check out the DLL4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DLL4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DLL4 Antibody Picoband® (A00875-4)
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